Structure and Function of Pig and Bovine Egg Zona Pellucida

1.1994(1)猪透明带的糖蛋白成分pzp3 α具有精子受体活性。o -糖苷链去除pzp3α后，pzp3α活性保留，而n -糖苷链去除pzp3α后，pzp3α活性丧失，表明猪精子与n -链结合。(2)三或四天线n -链是猪精子受体。(3)在牛带蛋白中发现了猪和小鼠带蛋白中不存在的高甘露糖型n链。(4)从牛带蛋白中分离出的n链精子受体活性很低。(5)猪带蛋白的3个组分与牛带蛋白表现出交叉反应的抗原性。2.1995(1)牛带蛋白的唾液酸在受精过程中被消除，导致酸性碳水化合物链中和。(2)牛ZPA蛋白在受精过程中发生特异性裂解。(3) bZPA成分中的半胱氨酸残基在受精过程中被氧化，导致带硬化。

1.1994(1) A glycoprotein component of pig zona pellucida, PZP3alpha, has sperm receptor activity. O-glycoside chain eliminated PZP3alpha retained the activity, while N-glycoside chain eliminated PZP3alpha lost the activity, indicating that boar sperm bind to the N-linked chain (s).(2) Tri-or tetra-antennnary N-linked chain was shown to be the pig sperm receptor.(3) The high mannose-type N-linked chains, which are absent from pig and mouse zona proteins, have been found in the bovine zona proteins.(4) The sperm receptor activity of the N-linked chains isolated from bovine zona proteins is shown to be quite low.(5) Three components of pig zona proteins showed cross-reactive antigenicity with respective bovine zona proteins.2.1995(1) Sialic acids of bovine zona proteins were shown to be eliminated during fertilization, resulting in neutralization of the acidic carbohydrate chains.(2) Bovine ZPA protein was shown to be cleaved specifically during fertilization.(3) Cysteine residues in bZPA component were shown to be oxidized during fertilization, inducing zona hardening.