500 MHz-NMR-Spektrometer with nitrogen-cooled inverse CryoProbe head

在萨尔兰大学，来自有机化学和药学系（用户社区NMR）的几个小组参与了复杂天然产物的分离和全合成。结构解析通常通过高分辨率质谱和NMR光谱的组合来完成。最后一项技术不仅对结构解析，而且对测定广泛存在于天然产物中的立体中心构型至关重要。此外，还将研究天然产物或潜在药物对其生物靶点的结合研究（STD-NMR）。利用现代反向冷冻探针技术，可以显着提高光谱仪的灵敏度，这在只有小样品探针可用时是重要的。所要求的反向探头是特别敏感的1H核，这是必不可少的结构和配置确定。到目前为止，用户群体还无法获得这样的探头。出于经济考虑，我们申请使用氮气冷却探头，而不是氦气冷却探头。所需的光谱仪是一台使用了25年的500 MHz光谱仪的替代品，该光谱仪必须在适当的时候更换。它所在的建筑将在今年夏天被拆除，由于它的非屏蔽磁铁，不可能在其他地方运行。此外，软件已完全过时，也不再提供备件。

At Saarland University several groups from the department of Organic Chemistry and Pharmacy (user community NMR) are involved in the isolation and total synthesis of complex natural products. Structure elucidation is generally accomplished via a combination of high resolution mass spectrometry and NMR spectroscopy. The last technique is essential not only for structure elucidation but also for the determination of the configuration of stereogenic centers, widespread found in natural products. In addition, binding studies of natural products or potential drugs towards their biological targets will be investigated (STD-NMR). With modern inverse cryoprobe technique the sensitivity of the spectrometer can be increased significantly, what is important if only small sample probes are available. The requested inverse probe head is especially sensitive for 1H nuclei, what is essential for structure and configuration determination. Such a probe head is not available to the user community so far. For reasons of economy we apply for a nitrogen- and not a helium-cooled probe head.The requested spectrometer is a substitution for a 25 year old 500 MHz spectrometer, which has to be replaced in due course. The building where it is located will be teared down this summer, and because of its unshielded magnet there is no possibility the run it somewhere else. In addition, software is completely obsolete and spare parts are not available any more.