High resolution inverted confocal laser-scan microscope with FCS

该方案旨在获得高分辨率共聚焦激光扫描显微镜、快速采集模式、大型孵育单元和用于荧光相关光谱测量的模块。一个大型的培养单元将确保使用活细胞进行长期实验的生理条件。FCS模块将允许测量蛋白质-蛋白质相互作用，进行共定位研究，并确定细胞内有效扩散常数。所设想的仪器还满足了传统共焦显微镜测量的需要，这些测量可以通过点扫描显微镜进行，因此也有助于减少目前可用的共焦系统的等待时间。所要求的显微镜将在技术平台“细胞分析”中集中操作。斯图加特研究中心系统生物学，因此是开放给所有成员和相关的工作组在整个大学。仪器的使用时间按第一年内2600小时计算。

This proposal aims at the akquisiton of a high-resolution confocal laser-scanning-microscope, fast acquisition mode, large incubation unit and a module for measurements by fluorescence correlation spectroscopy is applied for. A large incubation unit will ensure physiological conditions for long-term experiments using living cells. The FCS module will allow to measure protein-protein interactions, to perform co-localization studies and to determine intracellular effective diffusion constants. The envisaged instrument additionally covers needs for conventional confocal microscopic measurements that can be performed by point-scanning microscopy and will therefore also contribute to reducing waiting times for the presently available confocal system.The requested microscope will be operated centrally in the technology platform “Cellular Analytics” of the Stuttgart Research Center Systems Biology and is therefore open to all members and associated workgroups across the university. The time of usage for the instrument is calculated as 2600 hours within the first year.