Development of Bone-bonding Bioactive Titanium Using Nanotechnology and its' Application for Orthopedic Implant.

1.我们研究了表面带有纳米多孔锐钛矿晶体层的阳极氧化钛（AO Ti）的纳米孔结构、体外生物活性和体内骨结合能力。在1 M-H_2SO_4中以150V和200V阳极氧化AO Ti板，植入成熟兔胫骨近端跖骨4、8、16和24周，通过光镜、扫描电镜和分离实验观察。在植入后观察到高的骨结合能力，与我们之前的生物活性钛的研究数据相当。制备了多种含纳米二氧化钛（TiO2）颗粒的生物活性聚甲基丙烯酸甲酯（PMMA）基骨水泥，并对其力学性能和骨导电性进行了评价。市售PMMA水泥（PMMAc）作为对照。将骨水泥置入大鼠胫骨，原地固化。6周和12周后，取胫骨，采用扫描电镜（SEM）、接触显微放射照相（CMR）和吉姆萨表面染色评估骨传导能力。与市售的PMMA水泥相比，含有纳米金红石或锐钛矿的PMMA基水泥具有更好的骨传导能力。这些结果表明含有纳米二氧化钛颗粒的pmma基骨水泥是一种很有前途的骨替代品材料。我们研究了纳米级生物活性多孔表面与微尺度大孔结构之间的关系，并基于微聚焦x射线计算机断层扫描系统，对不同孔径和孔隙度（直径为6mm，长为15mm）的不同类型生物活性多孔钛植入物进行了三维互联性分析。使用相同的植入物植入公兔股骨髁6周和12周，进行体内组织形态学分析。该匹配分析揭示了宽互连（直径大于52 μm）对于骨长入具有纳米二氧化钛表面的多孔结构的重要性。少

1.We investigated nanoporous structure, in vitro bioactivity, and in vivo bone-bonding ability of anodically oxidized titanium (AO Ti) with a nanoporous anatase crystal layer on its surface. AO Ti plates, anodically oxidized at 150V and 200V in 1 M-H_2SO_4, were implanted into the proximal metaphyses of mature rabbit tibiae for 4, 8, 16, and 24 weeks and investigated by light microscopy, scanning electron microscopy and detaching test. High bone-bonding ability, comparable to our previous study data of the bioactive titanium, was observed during post implantation times.2.Various types of bioactive polymethylmethacrylate (PMMA)-based bone cement containing nano-sized titania (TiO2) particles were prepared, and their mechanical properties and osteoconductivity are evaluated. Commercially available PMMA cement (PMMAc) was used as a control. The cements were inserted into rat tibiae and allowed to solidify in situ. After 6 and 12 weeks, tibiae were removed for evaluation of osteoconductivi … More ty using scanning electron microscopy (SEM), contact microradiography (CMR), and Giemsa surface staining. The PMMA-baced cement with nanosized rutile or anatase showed superior osteoconduction compared to the commercially available PMMA cement. These results show that PMMA-based bone cement containing nanosized titania particles is a promising material for use as a bone substitute.3.We investigated the relationship between nanosized bioactive porous surface and micronsized macropore structure, various types of bioactive porous titanium implants with different pore sizes and porosities (6mm in diameter and 15mm long) were analyzed using specific algorithms for 3D analysis of interconnectivity based on a micro focus X-ray computed tomography system. In vivo histomorphometric analysis was performed using the very same implants implanted into the femoral condyles of male rabbits for 6 and 12 weeks. This matching study analysis revealed the importance of wide interconnections (diameter more than 52 μm) for bone ingrowth into porous structure with nanosized titania surface. Less