Wide field microscope for live cell microscopy

申请的装置将用于广泛的生物、医学和生物材料研究。有了这项技术，将首次有可能解决问题，特别是在生命系统(细胞、有机体、组织切片和生物材料)中，以前只有结合不同的技术才能做到这一点。现在可以在活体样品中拍摄高穿透深度和高分辨率的图像，非常快速(在空间和时间上)，并在长时间内对样品进行柔和处理。这将应用于不同物种(小鼠、人类)和来源(肠道、神经元、卵巢)的有机化合物，以在空间和时间维度上调查不同的问题。神经细胞器中的钙稳态和谷氨酸的释放、肠细胞器中的炎症过程或转录因子如SOX-11或TCF4在神经细胞器中的分布和调节。异种移植小鼠肿瘤组织切片的研究。植物中蛋白质分布的研究，即根、茎和叶。计划在生物材料研究领域对三维基质中的毛囊进行另一种应用。在不同的有机模型上建立屏障完整性分析是另一个重点。

The device applied for is to be used in a wide range of biological, medical and biomaterials research. With this technology, it will be possible for the first time to address questions, especially in living systems (cells, organoids, tissue sections and biomaterials), which were previously only possible by combining different technologies. It is now possible to take images in living samples with high penetration depth and high resolution, very fast (spatially & temporally) and gentle on the sample, over long periods of time. This will be applied to organoids of different species (murine, human) and origin (intestinal, neuronal, ovarian) to investigate different questions in spatial and temporal dimensions. Calcium homeostasis and glutamate release in neuronal organoids, inflammatory processes in intestinal organoids or the distribution and regulation of transcription factors such as Sox-11 or TCF4 in neuronal organoids. Studies in tumor tissue sections from xeno-graft mice. The investigation of protein distributions in planta, i.e. roots, stems and leaves. Another application is planned in the field of biomaterial research on follicles in three-dimensional matrices. The establishment of barrier integrity assays on different organoid models is another focus.