Nanoseond Ultra-Rapid Freeze Quenching for Time-resolved Structural Biology

该应用程序旨在开发用于生化过程的时间分辨研究的仪器，产生以原子分辨率实时记录的3D分子电影。应用的目标是常规和定制的设备，用于预稳态触发、采样、探测和检测，包括光学、低温沉积和超高真空系统、超高真空样品传输和计算集群升级。这种仪器对于时间分辨的结构生物学，特别是固态核磁共振和低温电子显微镜来说，将是必不可少的。

The application aims to develop instrumentation for the time-resolved studies of biochemical processes, yielding 3D molecular movies recorded with atomic resolution in real time. Application targets regular and custom-made equipment for pre-steady-state triggering, sampling, probing and detection, including optical, cryo-sedimentation and ultra-high vacuum systems, UHPLC sample delivery and the computation cluster upgrade. Such instrumentation will be essential for time-resolved structural biology, in particular solid state NMR and cryo-EM.