NMR console (replacement) for 600 MHz spectrometer, cryogenic cooling platform and 19F probe head.

该应用程序集成到2020年3月26日开始的Covid19-核磁共振项目中。在这个由来自10个国家的30多个合作伙伴组成的国际组织中，生产了SARS-CoV-2的所有病毒RNA和病毒蛋白，并对它们的核磁共振谱进行了分析和归属。生化产物和核磁共振光谱表征(核磁共振任务)构成了大型筛选项目的基础。此筛选项目的基础设施支持是当前应用程序的主题。Covid19-核磁共振的目标是筛选多达20种病毒蛋白和40种病毒RNA，以结合配体和药物。低分子化合物组装在五个不同的文库中，最多可达1000个配体。基于配体的筛选分为含19F原子的配体和不含19F原子的配体。基于19的筛查比基于1H的筛查具有许多优势。因此，我们申请将现有的600 MHz核磁共振谱仪升级为19F低温探头，允许1H去耦合。要做到这一点，我们需要更新一个已有20年历史的控制台和相关的低温冷却装置。对这一应用的评估是当务之急。生产公司布鲁克是提供这种设备的唯一公司，该公司已提出在2020年8月之前交付设备，以加快Covid19-核磁共振的研究。

The application is integrated into the project Covid19-NMR that started March 26th, 2020. In this international with more than 30 Partners from 10 countries, all viral RNAs and viral proteins of SARS-CoV-2 are produced and their NMR spectra analyzed and assignment. The biochemical production and the NMR-spectroscopic characterization (NMR assignments) forms the basis for a large screening project. The infrastructural support for this screening project is the topic of the current application. The aim of Covid19-NMR is to screen up to 20 viral proteins and 40 viral RNAs for binding of ligands and drugs. The low molecular weight compounds are assembled in five different libraries with up to 1000 ligands. Ligand-based screening is divided into ligands with 19F atoms and ligands without 19F. 19-based screening offers a number of advantages over 1H-based screening. Therefore, we apply for the upgrade of an existing 600 MHz-NMR-spectrometer with a 19F cryogenic probe allowing for 1H decoupling. To do so, we need to renew a 20 year old console and the associated cryogenic cooling unit. Evaluation of this applciation is urgent. The producing company Bruker, the sole company providing such eqiupment, has offered to deliver the equipment till August 2020 in order to expedite research in Covid19-NMR.