REQUEST TO PURCHASE A 600 MHZ NMR REPLACEMENT CONSOLE AND COLD PROBE

描述（由申请人提供）：华盛顿大学高分辨率核磁共振设施正在申请资金，为其核心600 mhz核磁共振仪器购买最先进的替代控制台和高端技术冷探针。新控制台和冷探针的结合将显著提高信号噪声和仪器稳定性，从而极大地促进正在进行的生物化学和生物医学研究项目，最终影响人类健康。该请求将使整个大学范围内的核磁共振设施的最高场光谱仪现代化，该设施服务于40个独立的研究小组，涵盖麻醉学、生物化学和分子生物物理学、生物学、生物医学工程、心脏病学、化学、传染病、内科、神经病学、眼科学、病理学、药理学和发育生物学、物理学和放射学（放射科学部）。核磁共振设施支持和维护六个仪器，并配备经验丰富的博士级专业人员，致力于用户培训和合作研究。设施是在它的

DESCRIPTION (provided by applicant): The Washington University High Resolution NMR Facility is requesting funds to purchase a state- of-the-art replacement console and a high-end technology cold probe for its core 600-MHz NMR instrument. The combination of new console and cold probe will markedly improve signal-to-noise and instrument stability, thus greatly facilitating ongoing biochemistry and biomedical research programs, ultimately impacting human health. This request will modernize the highest field spectrometer of the University-wide NMR Facility that serves 40 separate research groups, spanning departments of Anesthesiology, Biochemistry & Molecular Biophysics, Biology, Biomedical Engineering, Cardiology, Chemistry, Infectious Diseases, Internal Medicine, Neurology, Ophthalmology, Pathology, Pharmacology & Developmental Biology, Physics, and Radiology (Division of Radiation Sciences). The NMR Facility supports and maintains six instruments and is staffed with seasoned PhD-level professionals who are committed to user training and collaborative research. The Facility is in its 28th year of operation, is open and available 24 hours a day, 365 days a year, and is committed to serving its extensive user base. This funding request is prompted by the need to maintain the Facility's capabilities in the face of extraordinary technological advances over the past decade, to reduce instrument down-time and maintenance expense, and importantly, to add an ultra-high-sensitivity cold probe that will enable new experiments and provide experimental efficiencies via significantly enhanced signal sensitivity. The scientific research described in ths proposal spans user groups with longstanding interests in fundamental biological, biochemical, and biomedical research and clinically related health questions. These including research areas directed toward light initiated alterations of DNA structure, the creation of molecular patterns that mimic neural communication networks, understanding markers for the genesis and recognition of cancer, understanding structure and genesis of amyloid precursors, elucidation of tissue damage pathways caused by reactive oxidative molecules, and deciphering the altered metabolism of the diabetic heart as well as metabolism alterations associated with carbohydrate-poor diet. This request is made with a financial matching fund commitment from Washington University in the sum of $195,000.