REQUEST TO PURCHASE A 600 MHZ NMR REPLACEMENT CONSOLE AND COLD PROBE

描述(由申请人提供)：华盛顿大学高分辨率核磁共振设施正在申请资金，为其核心600-MHz核磁共振仪器购买最先进的更换控制台和高端技术冷探头。新的控制台和冷探头的结合将显著提高信噪比和仪器稳定性，从而极大地促进正在进行的生物化学和生物医学研究计划，最终影响人类健康。这一请求将使全大学核磁共振设施的最高场谱仪现代化，该设施服务于40个不同的研究小组，横跨麻醉学、生物化学和分子生物物理学、生物学、生物医学工程、心脏病学、化学、传染病、内科、神经病学、眼科、病理学、药理学和发育生物学、物理学和放射学(放射科学部)。核磁共振设施支持和维护六台仪器，配备了经验丰富的博士级别的专业人员，他们致力于用户培训和合作研究。该设施在其 运营第28年，一年365天、每天24小时开放，致力于为其广泛的用户群提供服务。这一资金申请是为了在过去十年中面对非凡的技术进步保持该设施的能力，减少仪器停机时间和维护费用，以及重要的是，增加一个超高灵敏度的冷探头，该探头将支持新的实验，并通过显著增强的信号灵敏度来提供实验效率。该提案中描述的科学研究跨越了对基础生物学、生化和生物医学研究以及临床相关健康问题具有长期兴趣的用户群体。这些领域包括针对光引发的DNA结构变化的研究领域，模拟神经通信网络的分子模式的创建，了解癌症发生和识别的标记，了解淀粉样前体的结构和起源，阐明由反应性氧化分子造成的组织损伤途径，以及破译糖尿病心脏的代谢变化以及与缺乏碳水化合物饮食相关的代谢变化。提出这一请求时，华盛顿大学承诺提供19.5万美元的财政配对基金。

DESCRIPTION (provided by applicant): The Washington University High Resolution NMR Facility is requesting funds to purchase a state- of-the-art replacement console and a high-end technology cold probe for its core 600-MHz NMR instrument. The combination of new console and cold probe will markedly improve signal-to-noise and instrument stability, thus greatly facilitating ongoing biochemistry and biomedical research programs, ultimately impacting human health. This request will modernize the highest field spectrometer of the University-wide NMR Facility that serves 40 separate research groups, spanning departments of Anesthesiology, Biochemistry & Molecular Biophysics, Biology, Biomedical Engineering, Cardiology, Chemistry, Infectious Diseases, Internal Medicine, Neurology, Ophthalmology, Pathology, Pharmacology & Developmental Biology, Physics, and Radiology (Division of Radiation Sciences). The NMR Facility supports and maintains six instruments and is staffed with seasoned PhD-level professionals who are committed to user training and collaborative research. The Facility is in its 28th year of operation, is open and available 24 hours a day, 365 days a year, and is committed to serving its extensive user base. This funding request is prompted by the need to maintain the Facility's capabilities in the face of extraordinary technological advances over the past decade, to reduce instrument down-time and maintenance expense, and importantly, to add an ultra-high-sensitivity cold probe that will enable new experiments and provide experimental efficiencies via significantly enhanced signal sensitivity. The scientific research described in ths proposal spans user groups with longstanding interests in fundamental biological, biochemical, and biomedical research and clinically related health questions. These including research areas directed toward light initiated alterations of DNA structure, the creation of molecular patterns that mimic neural communication networks, understanding markers for the genesis and recognition of cancer, understanding structure and genesis of amyloid precursors, elucidation of tissue damage pathways caused by reactive oxidative molecules, and deciphering the altered metabolism of the diabetic heart as well as metabolism alterations associated with carbohydrate-poor diet. This request is made with a financial matching fund commitment from Washington University in the sum of $195,000.