REQUEST TO PURCHASE A VARIAN HIGH RESOLUTION NMR REPLACEMENT CONSOLE

描述(由申请人提供)：华盛顿大学高分辨率核磁共振设施申请资金，为老化的500 MHz核磁共振仪器购买瓦里安DirectDriveT控制台，以及旋转NanoprobeT的魔角样品，以便研究癌症组织的代谢物特征。这些请求是为了补充和现代化共享的磁共振资源，该资源为38个独立的研究小组提供服务，这些研究小组横跨麻醉学、生物化学和分子生物物理学、化学、心脏病、工程学、内科、神经病学、眼科、病理学、药理学和分子生物学、物理学、放射肿瘤学和放射学。核磁共振设施是一个活跃的资源，容纳了五台仪器，并配备了致力于用户培训和合作研究的经验丰富的专业人员。该设施一年365天、每天24小时开放，并致力于为其广泛的用户群提供服务。这一要求是由于与可追溯至1986年的现有设备500 MHz仪器相关的大量停机时间和维护费用引起的。这台仪器二十多年来一直为数十名首席调查人员的研究需求服务，现在已经过时，不可靠。这一建议的科学基础跨越了在健康相关问题上长期感兴趣的用户群体。这些研究领域包括光引发DNA结构的改变，模拟神经通讯网络的分子结构的创建，了解癌症发生和识别的标记，生命系统中活性氧化分子造成的组织损伤，以及了解糖尿病心脏代谢的改变。这项314 395美元的请求是与华盛顿大学75000美元的财政承诺一起提出的。

DESCRIPTION (provided by applicant): The Washington University High Resolution NMR Facility requests funding to purchase a Varian DirectDriveT console for an aging 500 MHz NMR instrument and a magic angle sample spinning NanoprobeT to allow for studies of metabolite profiles from cancer tissues. These requests are for supplementing and modernizing a shared magnetic resonance resource that serves 38 separate research groups spanning departments of Anesthesiology, Biochemistry & Molecular Biophysics, Chemistry, Cardiology, Engineering, Internal Medicine, Neurology, Ophthalmology, Pathology, Pharmacology & Molecular Biology, Physics, Radiation Oncology, and Radiology. The NMR Facility is an active resource housing five instruments and staffed with seasoned professionals committed to user training and collaborative research. The Facility is open and available 24 hours a day, 365 days a year and committed to service for its extensive user base. This request is prompted by significant down time and maintenance expense associated with the existing Facility 500 MHz instrument that dates back to 1986. This instrument has served research needs for dozens of principal investigators for over two decades and is now dated and unreliable. The scientific basis for this proposal spans a user group with longstanding interests in health related issues. These including research areas of light initiated alterations of DNA structure, the creation of molecular structures that mimic neural communication networks, understanding markers for the genesis of and recognition of cancer, tissue damage caused by reactive oxidative molecules in living systems, and understanding the altered metabolism of the diabetic heart. This request, $314,395, is made with a financial matching commitment from Washington University in the sum of $75,000.