Analysis of primordial germ cells migration for infertility in Japanese flounder

在海水养殖鱼类中，原始生殖细胞PGC基本动态的分子机制尚未见报道。本研究以日本比目鱼为材料，对PGC的迁移及其分子机制进行了分析。cxcr 4（4型趋化因子受体）基因是从日本比目鱼中分离出来的，已知其与原始生殖细胞的迁移有关，本研究尝试用趋化因子受体抑制剂AMD3100特异性地结合cxcr 4，拮抗性地抑制PGC的迁移。结果，在施用AMD 3100的个体中，PGC标记vasa mRNA在日本比目鱼中消失。从这个结果可以清楚地看出，cxcr4参与了PGC在比目鱼中的迁移。

In marine farmed fishes, it is not yet investigated the molecular mechanism in fundamental dynamic state of primordial germ cell PGC. Then, in this study, migration of PGC and the molecular mechanism were analyzed using the Japanese flounder. The cxcr4 (4 type chemokine receptor) gene is isolated in Japanese flounder, which is known that the involved with migration of a primordial germ cell.Moreover, I tried prevention of PGC migration by using a chemokine receptor inhibitor AMD3100 which combines with cxcr4 specifically and inhibit the work antagonistically. As a result, in the individual which administrated AMD3100, a PGC marker vasa mRNA is disappeared in Japanese flounder. It became clear from this result that cxcr4 participates in migration of PGC in theflounder.