Development of Analysis Method of Immune Surveillance System in Various Animals by using Adenovirus Vector.

本研究的目的是利用腺病毒载体表征细胞介导的免疫监视系统，建立多种动物免疫监视系统的分析方法。结果表明：(1)建立了犬瘟热病毒（CDV）血凝素(H)基因重组腺病毒载体。我们还建立了犬皮肤原代细胞的制备方法，用于细胞毒性T淋巴细胞（CTL）试验。这使我们有可能从自我动物身上获得目标细胞。(2)确定了牛瘟病毒N蛋白细胞免疫和体液免疫的抗原表位。利用免疫刺激复合物（ISCOM），揭示了重组H蛋白在细胞免疫中有效。(3)鉴定出抗牛白血病的主要组织相容性复合体（BoLA）ⅱ类分子；BoLA-DR3等位基因。重组腺病毒编码BLV基因组

The AIM of this research is the development of analysis method of immune surveillance system in various animals by using adenovirus vector to characterize cell mediated immunity surveillance system. The various results were obtained, as followings,(1) We established the recombinant adenovirus vector encoding canine distemper virus (CDV) hemagglutinin (H) gene. We also established the method to get primary dog skin cells for the target of cytotoxic T lymphocyte (CTL) assay. It makes us possible to get the targeted cells from self-animals.(2) We identified epitopes for cell-mediated immunity and humoral immunity in N protein of rinderpest virus. By utilizing immune-stimulating complex (ISCOM), recombinant H protein was revealed to be effective in cell mediated immunity.(3) We identified the resistant Major Histocompatibility Complex (BoLA) class II molecules to the bovine leukemia ; BoLA-DR3 allele. The recombinant adenovirus encoding BLV genome