Structure and Function of Cell Surface Molecules

1 . Nakano和Yonezawa对猪和牛卵子表面透明带糖蛋白的精子配体碳水化合物链进行了表征。结果表明，位于ZPB n端区域的三/四天线复合物型链具有猪精子配体活性，而高甘露糖型链具有牛精子配体活性。此外，他们和Kiss还发现了一种α-甘露糖苷酶通过gpi锚点整合在猪精子质膜上。Takeo与Sipiczki合作研究了酵母的形态发生。酵母的形状大致由生长和分裂位置决定。质膜的超微结构与生长分裂有关。分裂酵母的分裂位点由细胞骨架和新旧细胞壁决定。诱导酵母-霉菌转化的环境因子因物种而异，但在物种之间观察到基本相同的细胞质响应。3: Aimi对Barabas实验室培养的灰黄链霉菌进行了化学研究。原始菌株来自科威特受石油污染的土壤。β-内酰胺酶抑制部分含量极低，无法进一步研究。利用质谱和核磁共振谱对具有抗生素活性的AK25和3-2组分进行光谱研究仍在进行中。4 . Oguma开发了一种流动注射法，在冠醚负载树脂柱上耦合预富集-火焰原子吸收光谱法测定铅。本文还研究了螯合树脂柱在线富集与石墨炉原子吸收光谱法相结合的方法，并将其应用于地下水和自来水中痕量镉的测定。建立了用石墨炉原子吸收光谱法测定人发中锡的固体样品法。

1 : Nakano and Yonezawa characterized the sperm ligand carbohydrate chains of pig and bovine egg surface zona pellucida glycoproteins. As a result, it has been revealed that tri-/tetra-antennary complex-type chains situated in the N-terminal region of ZPB possess pig sperm ligand activity, while a high mannose-type chain possesses bovine sperm ligand activity. Furthermore, they and Kiss found an α-mannosidase integrated in the pig sperm plasma membrane through GPI-anchor.2 : Morphogenesis of yeasts has been studied by Takeo in cooperation with Sipiczki. Shapes of yeasts were roughly determined by growth and division sites. Ultrastructure of the plasma membrane revealed correlation with growth and division. Division site in the fission yeast was determined by cytoskeletones and the old and the new cell walls. The environmental factors which elicited yeast-mold transformation were varied with species, but fundamentally equal cytoplasmic responses were observed among species.3 : Aimi carried out a chemical study for the cultured strain of Streptomycetes griseoflavus cultured in the laboratory of Barabas. The original strain was collected from the oil-polluted soil of Kuwait. The β-lactamase inhibiting fraction was minute in the amount and further study was not possible. Spectroscopic studies by use of mass and NMR spectra on fractions AK25 and 3-2 that have antibiotic activities are still under the way.4 : Oguma developed a flow injection method for determination of lead by coupling preconcentration on a crown ether loaded resin column with flame atomic absorption spectrometry. A combination of on-line preconcentration on a chelating resin column and graphite furnace atomic absorption spectrometry was also investigated and applied to determination of cadmium at a ppt level in underground water and tap water. Furthermore, a solid sample method was developed for determination of tin in human hair by graphite furnace atomic absorption spectrometry.