Induction of plant immunization by components of bio-resources and its application for screening of inducers.

已知植物通过系统信号传导获得在首先受到攻击的组织周围以及在远距离组织区域的诱导抗性。在植物中观察到的系统性氧化爆发，部分激发子具有诱导局部OXB和系统获得抗性（SAR）的活性，使我们计划这个项目。本课题的目的是分析系统性信号的调度和细胞间信号传导的生理机制，以激活系统性OXB，并将其作为SAR的诱导信号。真菌激发子和已知的SAR化学激活剂被证实诱导全身OXB和SAR。诱导系统性OXB的途径有两种，一种是通过真菌激发子激活局部OXB并在增强的组织细胞中产生过氧化氢，另一种是通过水杨酸和植物激活剂等刺激物激活非增强的细胞内部。3.在系统信号被翻译的组织细胞中，通过观察荧光指示剂来监测具有钙内流和pH增加的细胞的链反应。4.激发子负责基因克隆的差异显示植物组织处理后，激发子，并从使用的观点5。我们设计的系统性OXB诱导的基本机制的实验系统进行了改进，设置为一个手册，方便筛选植物免疫刺激剂或激活剂。6.利用该手册，我们从16科26种供试植物中筛选到了具有SAR诱导活性的OXB系统诱导剂

It is known that plants acquire induced resistance around firstly attacked tissue as well as at remote tissues area through a systemic signaling. An observation of systemic oxidative burst in plants which were partly stimulated by elicitor with activity to induce local OXB and systemic acquired resistance (SAR) had made us to plan this projects. The objective of this projects was to analyze the physiological mechanism of dispatching of systemic signals and signal transduction from cells to cells for activation of the systemic OXB which may become a induction signal for SAR.Results are as follows : 1. A fungal elicitor and known chemical activators of SAR were confirmed to induce systemic OXB and SAR.2. There was two ways to dispatch the systemic signal for induction of systemic OXB.One was through activation of local OXB and generated hydrogen peroxide in a potentiated tissue cells treated with fungal elicitor, the other through activation of non-potentiated inside cells treated with stimulators such as salicylic acid and plant activators. 3. In the tissue-cells where systemic signals were translated, a chain response of cells with calcium influx and pH increase was monitored by observations of fluorescence indicators. 4. Elicitor-responsible genes were cloned by a differential display from plant tissues following treatment with elicitor, and characterized from a viewpoint of usage 5. Our devised experimental systems for determination of fundamental mechanism of induction of systemic OXB were improved to set as a manual for convenient screening of stimulator or activator of plant immunization. 6. Using the manual, we obtained active systemic OXB inducers with SAR inducing activity from several plants of 16 families and 26 species of tested plants