Imaging and Manipulation of Single Living Cells with SECM

1.利用扫描电化学显微镜（SECM），通过绘制细胞周围局部氧浓度的二维图谱，对植物单个细胞的光合和呼吸活动进行成像. SECM用于研究化学物质对单个原生质体的影响。当DCMU（一种光合电子转移抑制剂）被注入原生质体时，由于原生质体产生的氧气减少，原生质体的SECM图像变得暗淡和变形。同样，动物细胞的呼吸活动也可以用SECM成像。双铂微盘电极被用作SECM的探针，同时检测两个电活性物种附近的一个单一的原生质体。根据Fe（CN）64-在一个圆盘上的氧化电流变化获得单个原生质体的地形图图像。在另一个圆盘上检测局部氧浓度用于成像细胞的光合活性.耗氧量 ...更多信息 用SECM技术对牛胚胎的氧耗率进行了无创性测定，当胚胎在体外受精后第6天达到桑椹胚（半径为74 μm）时，单个桑椹胚的氧耗率为1.40±0.27×10-14 mols-1。在SECM测量之后，将胚胎继续培养另外两天。囊胚的耗氧率为2.50±0.46×10-14 mol s-1<F<4.49±0.50×10-14 mol s-1.5。制作了一种用于生物检测的微生物芯片，并利用SECM技术对芯片上的大肠杆菌呼吸活性进行了表征。使用基于大肠杆菌的微生物芯片测量抗生素的杀菌效果。采用双微盘电极对单个藻进行介电电泳操作。根据施加在两个圆盘电极之间的交流电压的频率，诱导出一种吸引力或排斥力。基于介电电泳理论，研究了频率和溶液离子浓度对介电电泳力的影响。少

1. Scanning electrochemical microscopy (SECM) was used for imaging of photosynthetic and respiratory activity of a single plant cell by mapping two-dimensionally the localized oxygen concentration around the cell.2. SECM was used for investigation of the effect of chemicals on a single protoplast. When DCMU, a photosynthetic electron-transfer inhibitor, was injected into the protoplast, the SECM image of the protoplast becomes gloomy and deformed due to a decrease in the generation of oxygen from the protoplast Similarly, respiratory activity of animal cells can also be imaged with SECM.3. A dual-Pt microdisk electrode was used as a probe for SECM to simultaneously detect two electroactive species near a single protoplast. The topographic image of the single protoplast was obtained from the variation of oxidation current for Fe (CN) 64-at one disk. The detection of localized oxygen concentration at another disk was used for imaging photosynthetic activity of the cell.4. Oxygen consumpt … More ion of individual bovine embryos was noninvasively quantified by SECM.When an embryo reached the stage of a morula with a 74-μm radius on day 6 after in vitro fertilization, the oxygen consumption rate of the single morula was estimated to be 1.40±0.27×10-14 mol s-1. After the SECM measurement, the embryo was continuously cultured for another two days. The oxygen consumption rate of the blastocyst was found to be in the range of 2.50±0.46×10-14 mol s-1<F<4.49±0.50×10-14 mol s-1.5. A microbial chip for bioassay was fabricated and its performance was characterized by SECM.The respiration activity of the E.coli spot at the chip was imaged with SECM by mapping the oxygen concentration around the spot. The bactericide effects of antibiotics were measured using the E.coli-based microbial chip.6. Dielectrophoretic manipulation of a single chlorella was conducted using a dual-microdisk electrode. An attractive or repulsive force was induced to chlorella depending on the frequency of ac voltage applied between the two disk electrodes. The effect of both the frequency and ion concentration of solutions on the dielectrophoretic force on a chlorella was investigated in detail based on the theories of dielectrophoresis. Less