Isolation of a novel tumor suppressor gene on the Chromosome 11 associated with oral squamous cell carcinoma.

为了寻找与口腔鳞状细胞癌相关的肿瘤抑制基因(S)在11号染色体上的存在，我们对31例口腔鳞状细胞癌患者进行了该染色体上22个微卫星标记的高分辨率缺失定位。我们的数据显示了两个不同的、通常被删除的区域。第一个区域位于标记D11S939和D11S924之间，杂合性缺失频率较高，位于11q23的3厘米器官之间。在标记D11S912和D11S910之间发现了共同缺失的第二个区域，在11q25处相隔7 cM。我们的结果提示，在11号染色体的长臂上至少存在两个与口腔鳞癌发生有关的抑癌基因。为了确定位于染色体11q23和11q25的候选抑癌基因，采用差异显示法和代表性差异分析的方法，对这两个基因座上有或没有杂合性缺失的肿瘤进行了研究。共分离到4个DNA片段，其中1个位于染色体11q上。检测到该DNA片段的序列，被认为是参与口腔鳞癌发生发展的抑癌基因的一部分。

To search for the existence of tumor-suppressor gene(s) associated with oral squamous cell carcinoma (SCC) on chromosome 11, we have performed high-resolution deletion mapping in 31 patients with oral SCC using 22 microsatellite markers for this chromosomal region. Our data show 2 distinct, commonly deleted regions. The first region, with frequent loss of heterozygosity (LOH), was restricted between markers D11S939 and D11S924 separated by 3 centimorgans (cM) on chromosome 11q23. The second region of common deletion was identified between markers D11S912 and D11S910, separated by 7 cM at 11q25. Our results suggest tnat at least 2 tumor-suppressor genes involved in the development of oral SCC are present on the long arm of chromosome 11. To identify the candidate tumor suppressor genes at chromosome 11q23 and 11q25, differential display method and representational difference analysis, using tumors with and without LOH at these loci. Four DNA fragments were isolated and one of them was confirmed the presence on chromosome 11q. The sequence of the DNA fragment was detected and it was considered as a part of a putative tumor suppressor gene involved in the oncogenesis and development of oral squamous cell carcinoma.