Ion Trap mass spectrometer coupled to an HPLC including PC and nitrogen generator

离子陷阱质谱仪将主要用于初级和次级植物化合物的从头结构鉴定、已知物质的确认和这些植物化合物的定量。离子陷阱质谱仪的一个特别优点是分析物可以顺序地裂解成子离子(S)。多重碎裂(MSN)不能用任何其他类型的质谱计实现。离子陷阱质谱仪与高效液相色谱联用，适用于高通量样品，可用于多种项目，如：(1)复杂的黄酮苷和羟基肉桂酸衍生物的从头结构鉴定和定量。由于缺乏这类物质的复杂化合物的参照物，因此不可能只进行色谱分析。(2)对无参照物的类胡萝卜素立体异构体进行验证。在许多情况下，用质谱仪测量可以提供额外的信息，因为色谱方法不能提供足够的区分。只有在没有添加剂的APCI源的电晕放电针的帮助下，它们才有可能电离。(3)验证低浓度的叶绿素及其降解产物的结构建议。(4)保护硫代葡萄糖酸盐的拟议结构，这些硫代葡萄糖苷是健康保护性异硫氰酸酯或其他硫代葡萄糖酸盐降解产品的前体。(5)对于倍半萜内酯、生物碱和聚乙炔的新结构的阐明和验证，它们是植物中化学上不同的苦味物质。(6)用于皂苷的定性和定量分析，因为由于缺乏生色团，这些物质不能用层析法检测。(7)用于完整磷脂的分析，但尚未成功。由于化合物是同位素标记的，因此同位素峰的高分辨率是有利的。(8)不经衍生化或完全水解法分析多糖。顺序破碎可以从多糖中产生寡糖，这些寡糖可以用于分析，从而加快整体分析速度。在这些研究中，最高可达MS11的测量是可能的，并将进行测试。离子陷阱质谱仪安装在农业科学院的高效液相色谱-质谱仪服务单元中。

The ion trap mass spectrometer will primarily be used for the de novo structural elucidation of primary and secondary plant compounds, the validation of known substances and the quantification of these plant compounds. A particular advantage of the ion trap mass spectrometer is the sequential fragmentation of the analyte into daughter ion(s). Multiple fragmentation (MSn) cannot be achieved with any other type of mass spectrometer. The ion trap mass spectrometer is coupled to a HPLC, which is suitable for high sample throughput.The ion trap mass spectrometer is used in various projects, e.g. (1) for de novo structure elucidation and quantification of complex flavonoid glycosides and hydroxycinnamic acid derivatives. Due to the lack of reference substances for complex compounds of this group of substances, a chromatographic analysis only is not possible. (2) to validate carotenoid stereoisomers for which no reference substances are available. In many cases, measurement with a mass spectrometer can provide additional information in this case, since chromatographic methods cannot provide sufficient differentiation. Their ionization is only possible with the help of the corona discharge needle of an APCI source without additives. (3) to validate the structural proposals of chlorophylls and their degradation products, which occur in very low concentrations. (4) to protect the proposed structure of glucosinolates which are precursors for health-protective isothiocyanates or other glucosinolate degradation products. (5) for de novo structure elucidation and validation of the proposed structure of sesquiterpene lactones, alkaloids and polyacetylenes, which are chemically diverse bitter substances in plants. (6) for the analysis of saponins in terms of qualification and quantification, since these substances cannot be detected by chromatography due to the lack of chromophores. (7) for the analysis of intact phospholipids, which has not yet been successful. Since the compounds are isotopically labelled, a high resolution of the isotope peaks is advantageous. (8) for the analysis of polysaccharides without derivatisation or total hydrolysis. Sequential fragmentation can produce oligosaccharides from the polysaccharides, which can be used for analysis and thus speed up the overall analysis. For these investigations, measurements up to MS11 are conceivable and will be tested.The ion trap mass spectrometer is installed in the HPLC-MS Service Unit of the Faculty of Agricultural Sciences.