Identification creation of bioactive sites of enamel proteins for developing a new therapy of periodontal tissue regeneration.

为了鉴定被广泛应用于牙周再生治疗的Emdogain （EMD）的生物活性成分和活性位点，并探索利用其活性位点合成肽开发牙周组织再生新药的可能性，我们进行了以下研究。1.EMD促进牙周韧带细胞、牙龈上皮细胞、牙龈成纤维细胞和成骨细胞增殖和分化的细胞分子机制2.3. EMD的信号通路和受体；3 .确定EMD的有效成分和部位；活性位点合成肽对PDLC体外增殖分化及体内牙周组织再生的影响。研究结果为1。EMD刺激PDLC和成骨细胞的增殖和分化，抑制牙龈上皮细胞和成纤维细胞的增殖和分化2。RTK-ERK 1/2通路可能参与了PDLC对EMD的有丝分裂反应，3。3 .抗成釉酶抗体抑制EMD对PDLC增殖和分化的刺激作用。合成成釉酶活性部位的肽段可刺激PDLC的增殖、分化和牙周组织再生。综上所述，EMD对牙周组织再生具有良好的生物活性，本研究确定的合成肽可作为一种新的牙周组织再生治疗方法。

To identify the bioactive components and sites in Emdogain (EMD), which is widely used as a periodontal regenerative therapy, and to explore the possibility of developing new medicament using the synthetic peptide of the active sites for periodontal tissue regeneration, we achieved the following studies. 1.Cellular and molecular mechanism of stimulative effects of EMD on proliferation and differentiation of periodontal cells such as periodontal ligament cells (PDLC), gingival epithelial cells, gingival fibroblasts and osteoblasts. 2.Signal pathways and receptors of EMD, 3.Determination of active components and sites of EMD, and 4.Effects of the synthetic peptide of the active site on PDLC proliferation and differentiation in vitro and periodontal tissue regeneration in vivo. The results of the studies were 1.EMD stimulated proliferation and differentiation of PDLC and osteoblasts and inhibited those of gingival epithelial cells and fibroblasts, 2.RTK-ERK 1/2 pathway was possibly involved in mitogenic response of PDLC to EMD, 3.An anti-ameloblastin antibody suppressed stimulatory effects of EMD on PDLC proliferation and differentiation, and 4.The synthetic peptide of the active site of ameloblastin stimulated proliferation and differentiation of PDLC and periodontal tissue regeneration. In conclusions, EMD has suitable bioactivities to periodontal tissue regeneration and the synthetic peptide determined in the present study could be used as a new periodontal tissue regeneration therapy.