Development of Biomonitoring Technology for Environmental Endocrine Disruptors

环境内分泌干扰物对环境和农产品的污染是一个严重的全球性问题。这些化学物质在环境和农产品中以纳米级浓度被发现，并被怀疑影响生态系统和人类健康。传统的环境内分泌干扰物监测技术成本高、劳动强度大、耗时长，不能有效监测纳米水平的环境内分泌干扰物污染，因此需要开发新的技术来监测纳米水平的环境内分泌干扰物污染。(1)制备针对内分泌干扰物双酚A、壬基酚和除草剂甲草胺、辛马嗪的单克隆抗体，并基于该单克隆抗体建立酶联免疫吸附试验（ELISA）。与传统分析方法相比，该方法灵敏度高、操作方便、成本低。(2)以单克隆抗体为基础，通过基因工程技术制备重组scFv抗体，用于土壤和农产品样品提取物的亲和纯化。(3)发现结合亲和柱的新型ELISA方法可对各种样品中的环境内分泌干扰物产生高效的监测数据。

Contamination of the environment and agricultural products with environmental endocrine disruptors is a serious global problem. These chemicals were found in the environment and agricultural products at nano-level concentrations and suspected of affecting ecosystems and human health. Therefore, it is requested to develop novel technologies to monitor contaminations by nano-levels of environmental endocrine disruptors since most conventional technologies are high cost, labor intensive and taking time to obtain results, and do not work effectively to monitor nano-level contaminants.(1) We prepared monoclonal antibodies specific to the endocrine disruptors bisphenol A, and nonylphenol, and the herbicides alachlor and simazine, and then developed enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies. The ELISA methods developed were highly sensitive, convenient and low-cost as compared with the conventional analytical methods.(2) We also developed recombinant scFv antibodies based on the monoclonal antibodies by genetic engineering and used for preparation of affinity columns for clear-up of the extracts of samples of soil and agricultural products.(3) It was found that the combined use the novel ELISA method with the affinity column produced highly efficient monitoring data for environmental endocrine disruptors in a various samples.