Biochemistry of oil bodies in higher
Biochemistry of oil bodies in higher
批准号:
11694086
负责人:
HIRATA Toshifumi
金额:
$2.94万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B).
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2000
中文摘要
油体是植物细胞内物质积累的细胞器,本文对油体的超分子结构和功能进行了研究.预期母菊芽原基中的油体是由单分子的酰基甘油所包围。油体主要成分为三酰甘油、单酰甘油和二酰甘油、脂肪酸、磷脂和甾体.洋甘菊茎原基中的油体不含任何挥发性成分(精油),但含有少量蛋白质。洋甘菊种子油体中的蛋白质具有蛋白酶样活性,而芽原基油体中的蛋白质具有肽酶样活性.通过克隆甘菊油体中两种蛋白酶的cDNA,确定了其氨基酸序列,这两种蛋白均由501个氨基酸组成,分别具有丝氨酸羧肽酶和巯基蛋白酶的活性位点。还观察到,与这些肽酶相关的mRNA增加了化学应激对培养细胞的甘菊。
英文摘要
The supramolecule structure and function of oil bodies, which are known as an organelle for accumulation of the materials in the plant cells, were investigated.1. It was expected that the oil body in the shoot primordia of Matricaria chamomilla was constructed as a surrounded monolayer molecule of acylglycerols. The oil bodies contained triacylglycerols as major components, mono- and diacyl glycerols, fatty acids, phospholipids, and steroids.2. The oil body in the shoot primordia of M.chamomilla did not contain any volatile components (essential oils), but contained a small amount of proteins. Proteins containing in the oil body in the seeds of M.chamomilla have a protease-like activity, but those in the oil body of the shoot primordia showed a peptidase-like activity.3. Amino acid sequences of two proteases found in the oil bodies of M.chamomilla was determined by cloning of their corresponding cDNA ; both proteins are constructed by 501 amino acids, and have active cites for serine carboxypeptidase and thiol protease, respectively. It was also observed that mRNAs related to these peptidases were increased by the chemical stress against the cultured cells of M.chamomilla.
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Shinji Ohta: "Isolation and Identification of Cell Hypertrophy-Inducing Substances in the Gal Forming Aphid Colopha moriokaensis"Insect Biochem.Mol.Biol.. 30・10. 947-952 (2000)
Shinji Ohta:“Gal 形成蚜虫 Colopha moriokaensis 中细胞肥大诱导物质的分离和鉴定” Insect Biochem.Mol.Biol.. 947-952 (2000)。
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Toshifumi Hirata: "A 38 kDa Allylic Alcohol Dehydrogenase from the Cultured Cells of Nicotiana tabacum"Phytochemistry. 55. 297-303 (2000)
Toshifumi Hirata:“来自烟草培养细胞的 38 kDa 烯丙醇脱氢酶”植物化学。
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Toshifumi Hirata: "A 20-Kda protein mith the GTP-binding and trypsin inhibitory activities from Glycine max"Biosci. Biotechnol. Biochem.. 63・10. 1816-1818 (1999)
Toshifumi Hirata:“来自 Glycine max 的具有 GTP 结合和胰蛋白酶抑制活性的 20-Kda 蛋白质”Biosci. Biochem.. 1816-1818 (1999)。
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Chie Kohchi: "Isolation of a CDNA encoding for a carboxypeptidase, haning leucine zipper structure at the N-terminal region, from Matricaria chamomilla"Plant Biotechnol.. 16・5. 409-412 (1999)
Chie Kohchi:“从母菊中分离编码羧肽酶、N-末端区域的哈宁亮氨酸拉链结构的 cDNA”植物生物技术.. 16・5 (1999)。
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Toshifumi Hirata: "Epoxidation of Styrenes mith the peroxidase from the cultured cells of Nicotiana tabacum"Tetrahedron. 54. 15993-16003 (1998)
Toshifumi Hirata:“来自烟草培养细胞的过氧化物酶对苯乙烯的环氧化”四面体。
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共 16 条
Comparative Study on Citizenship Education and Education for ASEANness in ASEAN Countries
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批准号:22252007
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$21.88万
-
财政年份:2010
-
负责人:HIRATA Toshifumi
-
依托单位:
A Comparative Study on Citizenship Education in Thailand and Japan
-
批准号:14310129
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.1万
-
财政年份:2002
-
负责人:HIRATA Toshifumi
-
依托单位:
Scientific Research on Decentralization of Civic Education in Thailand
-
批准号:10610268
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.73万
-
财政年份:1998
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负责人:HIRATA Toshifumi
-
依托单位:
Chemical elucidation for the mechanism of the defense reaction of plant cells
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批准号:09480142
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.87万
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财政年份:1997
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负责人:HIRATA Toshifumi
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依托单位:
Biotransformation with the plant cultured cells-Clarification and development of its enantioselective reactions
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批准号:02804042
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1990
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负责人:HIRATA Toshifumi
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依托单位:
Histo pathological and clinical application of placental alkaline phostaphase as a tumor maker for primary intracranial germinoma.
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批准号:61570694
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$0.96万
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财政年份:1986
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负责人:HIRATA Toshifumi
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依托单位:
Fundamental and technological studies on the ability of the plant cultured cells to produce and transform organic compounds
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批准号:59840013
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项目类别:Grant-in-Aid for Developmental Scientific Research
-
资助金额:$5.82万
-
财政年份:1984
-
负责人:HIRATA Toshifumi
-
依托单位:
海外基金