Complicated falciparum malaria

1999年12月，我们访问了泰国和中国海南岛的三亚，调查了南亚地区严重恶性疟疾（SM）的情况，并采集了正常成人（NA）和恶性疟疾患者（Pf）的血液样本。我们参加了“第二届国际热带病会议”，收集了岛上和华南地区SM的最新信息。我们访问了曼谷玛希隆大学热带病医院，并在与缅甸接壤的一个地区获得了1000份来自NA和Pf的冷冻血液样本和10份新鲜Pf血液样本。同时也获得了他们的临床资料。血浆样本进行前列腺素分析，全血样本进行补体受体1 （CR1）基因分析，明确疟疾严重程度的遗传因素。我们访问了曼谷、清迈和与缅甸接壤的地区，以调查泰国的疟疾情况并获取疟疾患者的血液样本（2000年12月）。我们参加“第三届食源性人畜共患病研讨会暨国际热带医学联合会议”，收集泰国SM情况的最新资料，并在热带疾病医院取得1,500份疟疾患者和NA的血液样本及其临床资料。我们访问了清迈大学卫生科学研究所和清迈疟疾中心，调查了泰国北部的SM情况。大多数来自缅甸的难民感染了疟疾寄生虫。我们访问了好爱的疟疾控制中心，最近又进驻了热带医学培训中心，调查缅甸附近地区的疟疾情况。约90%的缅甸难民感染了疟疾寄生虫。收集的血浆样本用于前列腺素分析。部分血浆标本用于疟疾抗原检测，应用噬菌体展示法对疟疾进行诊断。全血样本用于CR1基因分析。

We visited Thailand and Sanya at the Hainan Island, China to investigate severe falciparum malaria (SM) situations at South Asia and to obtain blood samples from normal adults (NA) and falciparum malaria patients (Pf)(December 1999). We attended "the Second International Meeting on Tropical Diseases" to collect the latest information on SM at the island and South China. We visited Hospital for Tropical Diseases, Mahidol University at Bangkok and gained 1,000 frozen blood samples from and NA and Pf, and 10fresh Pf blood at an area bordering Myanmar. Their clinical data were also obtained. The plasma samples were used for prostanoid analysis and the whole blood samples were used complement receptor 1 (CR1) gene analysis to clarify genetic factor for malaria severity.We visited Bangkok, Chiang Mai and areas bordering Myanmar to investigate malaria situation at Thailand and to gain blood samples of malaria patients (December of 2000). We attended "The Third Symposium on Food-Borne Zoonoses and Joint International Tropical Medicine Meeting" to collect the latest information on SM situation on Thailand and gained 1,500 blood samples of malaria patients and NA and their clinical data at Hospital for Tropical Diseases. We visited the Institute of Health Science, Chang Mai University and Malaria Center at Chiang Mai to investigate SM situation at north Thailand. Most of refugees from Myanmar were infected with malaria parasites. We visited the Malaria Control Center at Haoyai and recently settled Training Center for Tropical Medicine to investigate malaria situation at near Myanmar areas. About 90% of refuges from Myanmar were infected with malaria parasites. The collected plasma samples were used for prostanoid analysis. A part of plasma samples were used for detection of malaria antigen with the use of phage display method to apply to malaria diagnosis. The whole blood samples were used for CR1 gene analysis.