Establishment of antigen-specific regulatory T cell lines induced by oral tolerance and the application to immunotherapy

口服耐受是一种很有前途的免疫治疗方法。由口服抗原产生的调节性T细胞将是最负责抑制作用的细胞群。为了建立调节性T细胞系，了解调节性T细胞的机制以及产生这些T细胞的条件是很重要的。因此，我们建立了一种检测系统，通过将候选调节细胞与抗原特异性脾细胞混合来监测对正在进行的免疫反应的抑制活性。我们接下来关注的是分泌tgf -的Th3/Tr1细胞，因为这种细胞是最具特征的调节细胞之一。因此，我们描述了几种产生Th3/Tr1细胞的培养条件。在不同条件下培养OVA特异性TCR转基因小鼠的脾脏细胞，并检测由此产生的OVA特异性细胞的tgf - β分泌或上述建立的评价体系。我们现在正在缩小产生调节细胞的合适条件。

Oral tolerance is one of the promising methods for immunotherapy. Regulatory T cells generated by the orally administered antigens would be the most responsible cell population for the suppressive action. In order to establish regulatory T cell lines, it is important to know the mechanisms of the regulation as well as the conditions to generate these T cells. We thus establish an assay system to monitor the suppressive activity to the ongoing immune-response by mixing the candidate regulatory cells with antigen-specific splenocytes. We next focused on the Th3/Tr1 cells which secrete TGF-beta because this cells is one of the most well characterized regulatory cells. We have thus characterize the several culture conditions to generate Th3/Tr1 cells. Spleen cells from OVA specific TCR transgenic mice were cultured in different conditions and resultant OVA specific cells were assayed for the secretion of TGF-beta or the above established evaluation system. We are now narrowing down suitable conditions for the generation of regulatory cells.