Organogenesis of established ES cells and its application for clinical medicine.

从大鼠早期胚胎干细胞（REEES）中建立了具有肝细胞特性的细胞系。该细胞系是采用美国等的一种新方法，从自发性侏儒大鼠（SDR）的两细胞胚胎中建立的。肝细胞系（REES-hep）是在胚胎发生过程中，使用在胚胎营养因子存在下培养的REES细胞系，从胚胎中的暗红色组织中建立的。这些细胞系用补充10%FBS和lng/ml LIF的DMEM/F12培养基培养。结果表明，它们保持二倍体状态，具有42条正常染色体，并增殖至融合，同时存在接触抑制。当使用胶原海绵凝胶系统培养时，这些细胞产生白蛋白，并以类似于肝细胞索的索状形式重建。当移植到SDR的脾脏中时，细胞还产生白蛋白和胆红素。此外，REES-hep细胞移植到肝功能不全大鼠的脾脏中促进其寿命的延长。从早期胚胎干细胞重建REES-hep细胞系有助于治疗肝功能不足的患者。为细胞移植及在生物混合型肝脏装置中的应用提供了参考。

A cell line with the characteristics of hepatocytes was established from rat early embryonic stem cells (REEES). This cell line was established using a new novel method of us et al. from two cell embryos taken from the spontaneous dwarf rat (SDR). The hepatocyte cell line (REES-hep) was instituted from dark red colored tissue in embryos during embryogenesis using REES cell line cultured in the presence of embryotrophic factors. These cell lines were cultured with DMEM/F12 medium supplemented 10% FBS and 1ng/ml of LIF. They were found to maintain their diploid state, were characterized with 42 normal chromosomed and proliferated to confluence, contact inhibition was also present. These cells produced albumin when cultured using a collagen sponge gel system and reconstructed in a funicular form resembling the cell cords of liver. The cells also produced albumin and bilirubin when transplanted into the spleen of SDR. Also, transplantation of REES-hep cells into the spleen of liver-insufficient rat promoted prolongation of its life. Reconstruction of a REES-hep cell line from early embryonic stem cells should help in treating hepatic insufficient patients. It will be valuable for further research, as an introduction to cell transplantation and application for use in a bio-hybrid typed liver apparatus.