Fundamental study for pathophysiology of human prostate hyperplasia in respect to gene expression profiles of proliferation/apoptosis related genes

DNA微阵列技术为我们提供了用少量样本分析多个基因的基因表达谱的方法。我们进行了DNA微阵列分析，了解前列腺疾病的病理生理。激光捕获显微切割（LCM）能够分别切割上皮和间质，我们使用LCM。在上皮成分中，E-cadherin、丝氨酸蛋白酶抑制剂、CD 9、法尼基转移酶、细胞周期蛋白2和细胞周期蛋白1的基因表达水平显著升高。另一方面，免疫球蛋白λ基因座、结缔组织生长因子、I型胶原、层粘连蛋白α 4、整合素α 7、TGF-β诱导的（68 kDa）的基因表达水平显著增加。进行了前列腺癌细胞基因表达谱分析的进一步研究。研究了染料木黄酮或己烯雌酚处理人前列腺癌细胞后的分子特征。染料木黄酮诱导谷胱甘肽过氧化物酶基因表达，我们认为染料木黄酮的抗肿瘤作用可能与抗氧化作用有关。

DNA microarray technology provides us to analyze gene expression profiles of many genes using a small amount of samples. We performed DNA microarray analysis for understanding of pathophysiology of prostate diseases. Laser capture microdissection (LCM) enables to dissect epithelium and stroma separately, and we utilized LCM. In epithelial component, gene expression levels of E-cadherin, serine protease inhibitor, CD9, farnesyltransferase, cyclin 2 and cyclin 1 were increased significantly. On the other hand, gene expression levels of immunogloburin lambda locus, connective tissue growth factor, collagen type I, laminine alpha4, integrin alpha7, TGF-beta-induced (68 kDa) were significantly increased. Further studies for analysis of gene expression profiles of prostate cancer cells were performed. Molecular characterization after genistein or diethylstilbestrol treatment in human prostate cancer cells was investigated. Genistein induced glutathione peroxidase gene expression, and we thought that anti-tumor effect of genistein involved anti-oxidant effects.