Nerve induction on the Titanium implant coated by essential trace elements

本研究是为钛种植体增加感觉功能的基础研究而设计的。在第一阶段，我们进行了细胞培养实验。但我们无法建立在钛表面上制作合适涂层的技术。第二阶段，进行机械结构分析，寻找神经纤维存在的结构要求。细胞培养：选用来源于大鼠肾上腺髓质棕色细胞的PC12细胞。我们将细胞培养在镀金的培养皿中。结果是PC12细胞不会发光金色。必需微量元素：我们尝试用离子溅射法等方法在钛表面添加必需元素。但很明显，这些标本不适合进行这项实验。力学结构分析：采用有限元方法对种植体和骨的结构进行力学建模。结果表明，种植体与种植体的分离区是位移最大的部位。这说明机械传感器不附着在植入物上更有效。种植体底部以下2.5~5.0 mm处总能量密度也最大。结构要求：神经细胞需要被力变形，才能检测到感觉感受器中的任何力。根据这项研究的结果，力感受器在分离的位置将比在种植体附着的位置更有效。在骨整合状态下，如果感觉感受器存在于骨和种植体的交界处，那么力的检测潜力就会很低。

This study was designed for the basic study to add sensory functions on the Titanium Dental Implants. In the first stage we made the cell cultivating experiments. But we couldn't establish technique to make a suitable coat upon Titanium surface. In the second stage, we made mechanical structure analysis and searched for the structural requirements of the existence of neural fibers.1. Cell cultivation : we used PC12 cell that have origin of the brown cell of adrenal medulla in rat. We cultivated the cell in the cultivating dishes coated by gold (Au). The result was that the PC12 cells would not glow with gold.2. Essential trace elements : We tried to add essentiol elements on the surface of Titanium using Ion-spattering method and others. But it became clear that the specimen were not suitable for this experiment.3. Analysis of the mechanical structure : We made a mechanical model for the structure with implant and bone using Finite Element Method. In the result, it was concluded that the place that shows the largest displacement is separated area from the implant body. This shows that it is more effective for the mechanical sensor not to be attached to the implant. The total energy density also was the maximal value in the place 2.5-5.0mm below the bottom of the implant.4. Structural requirements : It is necessary to be deformed by the force for neural cell to detect any force in sensory receptors. By the results of this study, the force sensory receptors will be more efficient in separated place than in attached place to the implant. In the state of Osseous integration, if the sensory receptors exist in the border between the bone and the implant, the detective potential for the force will be low.