Clinical application and functional analysis of MAGE E1 gene, glioma specific new gene belonging MAGE family

MAGE-E1是黑素瘤相关抗原基因（法师）家族的成员，其在恶性胶质瘤中相对于正常中枢神经系统细胞上调。本论文中，我们鉴定了MAGE-E1蛋白，并研究了其在胶质瘤细胞中的表达。在用匹配MAGE-E1蛋白片段的14-mer肽免疫的三只兔中产生抗体。Western blot分析和免疫细胞化学结果显示该蛋白定位于U87 MG细胞的胞浆中。在U87 MG细胞中，部分细胞核周围有蛋白质富集。MAGE-E1蛋白表达在10%胎牛血清培养的T98 G细胞中较高，但在血清饥饿72小时的生长停滞细胞中较低。用20%FBS刺激生长停滞的细胞，表达上调。从患者切除的胶质瘤的蛋白质印迹分析和免疫组化显示MAGE-E1蛋白在胶质母细胞瘤中的水平高于弥漫性星形细胞瘤。我们的研究结果表明，MAGE-E1在胶质瘤细胞的增殖中发挥作用。MAGE-E1可能成为恶性胶质瘤治疗的靶分子。

MAGE-E1 is a member of the melanoma-associated antigen gene (MAGE) family that is up-regulated in malignant glioma relative to normal central nervous system cells. In this manuscript, we identified MAGE-E1 protein and studied its expression in glioma cells. Antibodies were produced in three rabbits immunized with a 14-mer peptide that matched a MAGE-E1 protein segment. Western blot analysis and immunocytochemistry of U87MG cells showed the protein to be localized in the cytoplasm. The protein was enriched around the nucleus in some of U87MG cells. MAGE-E1 protein expression was high in T98G cells cultured with 10% fetal bovine serum but low in growth-arrested cells that were serum-starved for 72 hours. The expression was up-regulated by stimulation of growth-arrested cells with 20% FBS. Western blot analysis and immunohistochemistry of gliomas resected from patients showed MAGE-E1 protein to be present at higher levels in glioblastomas than in diffuse astrocytomas. Our results suggest that MAGE-E1 play a role in the proliferation of glioma cells. MAGE-E1 might be a target molecule for treatment of malignant gliomas.