Manual making of investigation method for maintenance with a molecule marker

维护生物多样性似乎是在生物多样性国家战略中指出的，它是自然环境保护的一大支柱之一。当我出于需要发展种群时，有必要根据种群的遗传模式来评估数量影响，以将对种群的影响保持在最低限度。因此，我在当地申请的七花之一的秋天，打算分布的白玉兰，蒲公英是一种而且普遍在紫茎泽兰和艾池周围，晚年数量急剧减少而有它的基本构成级，并给予一个濒临灭绝的河床植物物种和我使用的通用分子标记当代群结构分析的方法，调查和阐明了一组不同植物的不同程度的基因，目的是从过去的调查中制作一份共性的分子群分析手册，为维护提供分子数据和应用人员。关东区北仓区。关于一棵白玉兰，我用了我之前取样的东西。因此，对于毛木兰来说，在一个群体中和群体之间的专业度都很大。2003年，我新采集了一株蒲公英。此外，利用2002年前提取的全部DNA样本，对其进行了直接序列分析、微卫星测序、随机扩增多态性DNA分析，并根据分析结果制作了分子手册。

Maintenance of a biodiversity seems to be pointed out in a biodiversity nation strategy, and it is one of a big pillar of natural environmental safeguard. It is necessary to evaluate quantitative influence on the basis of hereditary pattern of population to keep influence for the kind within a minimum when I develop it out of necessity. Therefore application people are counted among one of the seven flowers of autumn and I am local and intend for a distributed Magnoria tomentosa, the dandelion which are a kind moreover generally around Eupatorium fortunei and Aichi that in late years the number decreases sharply while there is it with basic constitution class, and is given to an endangered species of vegetaition of a riverbed and I use present age group structure analysis method by a used general molecule marker and investigate it and clarify gene various degrees of a group of various plants and aim at making of a commonness molecule group analysis manual for maintenance from the past investigation that provided molecule data and application person did.2002The Eupatorium fortunei sampled it in Chubu District, Hokuriku District, Kanto District. About a Magnoria tomentosa, I used the thing which I sampled before. As a result, as for the Magnoria tomentosa, in a group and specialization between groups were big. For it, the Eupatorium fortunei had a small variation in a group.2003I collected a dandelion newly. In addition, with the all DNA sample which I extracted by 2002, I analyzed a direct sequence of a specification domain, a microsatellite, RAPD method and made a molecule manual from the result.