The influence of chromosome polymorphisms of Ranunculus silerifolius (Ranunculaceae) to genetic diversity of populations

利用等位酶多态性分析，揭示了部分繁殖分离的毛绒草Otaru和Matsuyama细胞型之间的基因流动。通过MDH-3等位基因的不同等位基因，可以区分邻群混合群体中的Otaru和Matsuyama细胞型，并且两种细胞型之间的杂交具有两种细胞型的两种等位基因。MDH-3基因座的等位基因分布模式表明，MIX内各细胞型之间不存在基因流动。两种细胞类型间的基因流动值(0.503)小于相同细胞类型的异地群体间的基因流动值(2.550或1.700)。群体间的遗传距离远大于相同细胞型的远距离群体间的遗传距离。利用等位酶标记的变异进行Nei‘s遗传距离的聚类分析表明，小太郎和松山的细胞型形成了…利用等位酶分析技术，对兼性自交物种毛绒草在95个群体中的遗传变异进行了研究，发现种内染色体具有Karatsu-、Matsuyama-、Mugi和Otaru-细胞型。对9个酶系统的16个基因座进行了解析。3个基因座在所有群体中都是单态的。平均而言，26%的座位是多态的，16个座位共扩增出49个等位基因，平均每个座位有1.29个等位基因。总体来说，遗传变异水平较低，与其他兼性自交物种一样。群体间的基因分化系数G&lt；ST&gt；较高(0.579)，可能反映了林木的近亲繁殖。遗传距离和地理距离之间没有相关性可能是由同样的影响造成的。居群间的平均遗传距离较大(D=0.129)，表明居群间分化程度较高，基因流动水平较低(Nm=0.171)，而细胞型间的遗传距离很小(D=0.008)，这可能是由于自细胞型出现以来遗传多样性的精细时期较短所致。较少

Gene flow between partially reproductively isolated Otaru and Matsuyama cytotypes of Ranunculus silerifolius was revealed using allozyme polymorphisms. Otaru and Matsuyama cytotypes in the parapatric population MIX were distinguishable critically by the different alleles of the Mdh-3 allozyme locus, and the hybrids between both cytotypes shared both alleles of both cytotypes. The distribution pattern of the alleles of the Mdh-3 locus revealed that the gene flow did not occur between cytotypes within MIX. The value of gene flow between the two cytotypes within the parapatric population MIX (i.e.0.503) was smaller than those between allopatric populations of the same cytotype (i.e.2.550 or 1.700). The genetic distance between cytotypes within the parapatric population MIX was larger than those between the far distant populations of the same cytotypes. Dendrograms based on the Nei's genetic distance using the variations of allozyme markers showed that Otaru and Matsuyama cytotypes formed … More clusters separately.Genetic variations of the facultatively autogamous species Ranunculus silerifolius, which showed intra-specific chromosomal polymorphisms (Karatsu-, Matsuyama-, Mugi, and Otaru-cytotype), were investigated in 95 populations using allozymic analysis. Sixteen loci of nine enzyme systems were resolved. Three loci were monomorphic for all populations. On average, 26% of the loci were polymorphic ; the 16 loci resolved gave a total of 49 alleles with a mean number of 1.29 alleles per locus. Overall levels of genetic variation were low as with other facultatively autogamous species. The gene differentiation coefficient among populations, G_<ST> was high (0.579), which probably reflected the inbreeding in R.silerifolius. The absence of a correlation between genetic distance and geographical distance may have resulted from the same effect. The average of genetic distance between populations was large (D=0.129), indicating a high level of differentiation and low level of gene flow (Nm=0.171) among the populations, while that between cytotypes was very small (D=0.008), possibly due to the relatively short period of fine for genetic diversification since the occurrence of the cytotypes. Less