Molecular biological Study of Muscle strengthening-enhancement for transcription by rehabilitation approach

本研究的目的是阐明中枢神经系统可塑性与各种刺激之间的相关性，这些刺激是中枢神经系统功能障碍恢复的主要机制。中枢神经系统的可塑性对脑卒中等中枢神经系统疾病的恢复及病变的愈合起着重要作用。Kaas报道了灵长类动物皮层损伤后，皮层重组使功能恢复。Nudo等指出，麻痹手指的恢复是通过手指的重复运动和皮层手指运动区的扩大来实现的。Ikeda指出，脊髓损伤后即刻，皮质转录因子的活性增强。然而，没有研究哪种刺激对功能恢复更好。本研究采用玫瑰红光敏染料制作大鼠脑梗死模型，用560 nm绿色光照射大鼠感觉-运动皮层，通过Beam Walking实验评价脑梗死后大鼠感觉-运动功能的恢复。然后我们检测了神经营养因子GDNF在梗死皮质的表达。功能恢复期梗死灶周围GDNF表达明显增加。在此基础上，研究康复运动对脑梗死偏瘫大鼠的影响。运动组与非运动组之间有显著性差异。此外，复杂运动组的恢复明显早于简单运动组。这些结果提示，适当的运动对功能恢复有更大的作用。

Aim of this study is to clarify the correlation between plasticity of central nervous system and various stimulation concerned as the main mechanism of functional recovery from dysfunction of central nervous system. The plasticity of central nervous system play a important role on the recovery of central nervous disease like stroke as well as heeling of lesion. Kaas reported that cortical reorganization made functional recovery after cortical injury on primate. Nudo et al. indicated the recovery of paralytic finger is obtained from repetitive exercise of the finger with enlargement of cortical finger motor area. Ikeda indicated cortical activity of transcription factor was enhanced immediate after spinal cord injury. However, there is no study about which stimulation is better on functional recovery. This study is to investigate the nature of stimulation which enhances the recovery on central nervous disease like stroke or spinal cord injury and to obtain mere recovery on clinical rehabilitation.We made cerebral infarction model using photosensitive die Rose-bengal and irradiate the 560nm green light on rat sensori-motor cortex and evaluated the functional recovery by Beam Walking test. Then we examined the expression of neurotrophic factor GDNF on the infarcted cortex. The expression of GDNF is significantly increased on the surrounding infarction at the period of functional recovery. Then we investigated the rehabilitative exercise effects on the hemiplegic infarcted rat. There is significant change between the exercise group and the non-exercise group. Additionally, complex exercise group recovered significantly earlier than simple exercise group. These findings suggested that appropriate exercise has more effects to functional recovery.