Mechanism of SARS coronavirus infection and proliferation

SARS冠状病毒感染和增殖机制

• 批准号: 21590517
• 负责人: TANAKA Nobuyuki
• 金额: $ 2.91万
• 依托单位: 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011)Miyagi Cancer Center Research Institute (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590517/
• 关键词: SARS; ウイルス; 共受容体; 細胞内侵入; コロナウイルス; ACE2; 偽ウイルス; スパイク

SARS-CoV attaches to the cell surface ACE2, however, requirement of additional cell surface proteins need to be clarified. In this study, we investigated the nature of co-receptors of SARS-CoV. By utilizing soluble SARS-CoV spike protein and a chemical crosslinker reagent, we identified two L-type lectins, DC-SIGN and L-SIGN, in the Spike-ACE2 complex. ACE2 forms complex with these proteins prior to the addition of the Spike. Although neither DC-SIGN nor L-SIGN increased SARS-CoV pseudovirus adsorption to the cell surface, these lectins facilitated incorporation of SARS-CoV in to the cells. Intracytoplasmic region of DC-SIGN and L-SIGN, but not that of ACE2, was required for the incorporation. These results suggest that the intracytoplasmic regions of DC-SIGN and L-SIGN play roles on SARS-CoV infection.

SARS-CoV结合在细胞表面的ACE2上，但需要更多的细胞表面蛋白，需要明确。在这项研究中，我们研究了SARS-CoV的协同受体的性质。利用可溶性SARS冠状病毒刺突蛋白和化学交联剂，我们在刺突-ACE2复合体中鉴定出两个L类型的凝集素DC-SIGN和L-SIGN。在添加Spike之前，ACE2与这些蛋白质形成复合体。虽然DC-SIGN和L-SIGN都不能增加SARS-CoV假病毒在细胞表面的吸附，但这些凝集素促进了SARS-CoV进入细胞内。DNA掺入需要DC-SIGN和L-SIGN的胞质内区，而不需要ACE2的胞质内区。这些结果提示DC-SIGN和L-SIGN胞质内区在SARS冠状病毒感染中起作用。

项目成果

Angiotensin II Type 1 Receptor Expression and Microvessel Density in Human Bladder Cancer

• DOI: 10.1016/j.urology.2010.11.002
• 发表时间: 2011-04-01
• 期刊: UROLOGY
• 影响因子: 2.1
• 作者: Shirotake, Suguru;Miyajima, Akira;Oya, Mototsugu
• 通讯作者: Oya, Mototsugu

Autophagy-dependent degradation of MARCH-VIII regulates MHC class II

MARCH-VIII 的自噬依赖性降解调节 MHC II 类

• 发表时间: 2009
• 作者: 磯野法子;田中伸幸;等
• 通讯作者: 等

Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein

• DOI: 10.1016/j.bbrc.2010.07.083
• 发表时间: 2010-08-27
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Tamai, Keiichi;Tanaka, Nobuyuki;Sugamura, Kazuo
• 通讯作者: Sugamura, Kazuo

Effects of acute gamma-irradiation on community structure of the aquatic microbial microcosm

急性伽马射线照射对水生微生物群落结构的影响

• 发表时间: 2010
• 期刊: J Environ Radioact
• 作者: Fuma S;Ishii N;Tanaka N;他(8人中7番目)
• 通讯作者: 他(8人中7番目)

Regulation of interleukin-21 receptor expression and its signal transduction by WSB-2

WSB-2对白细胞介素21受体表达及其信号转导的调控

• DOI: 10.1016/j.bbrc.2010.01.004
• 发表时间: 2010
• 期刊: Biochem. Biophys. Res. Commun
• 作者: Nara H;Onoda T;Rahman M;Araki A;Juliana FM;Tanaka N and Asao H
• 通讯作者: Tanaka N and Asao H