Mechanism of SARS coronavirus infection and proliferation

SARS冠状病毒感染和增殖机制

• 批准号: 21590517
• 负责人: TANAKA Nobuyuki
• 金额: $ 2.91万
• 依托单位: 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011)Miyagi Cancer Center Research Institute (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590517/
• 关键词: SARS; ウイルス; 共受容体; 細胞内侵入; コロナウイルス; ACE2; 偽ウイルス; スパイク

SARS-CoV attaches to the cell surface ACE2, however, requirement of additional cell surface proteins need to be clarified. In this study, we investigated the nature of co-receptors of SARS-CoV. By utilizing soluble SARS-CoV spike protein and a chemical crosslinker reagent, we identified two L-type lectins, DC-SIGN and L-SIGN, in the Spike-ACE2 complex. ACE2 forms complex with these proteins prior to the addition of the Spike. Although neither DC-SIGN nor L-SIGN increased SARS-CoV pseudovirus adsorption to the cell surface, these lectins facilitated incorporation of SARS-CoV in to the cells. Intracytoplasmic region of DC-SIGN and L-SIGN, but not that of ACE2, was required for the incorporation. These results suggest that the intracytoplasmic regions of DC-SIGN and L-SIGN play roles on SARS-CoV infection.

SARS-COV附着在细胞表面ACE2上，需要阐明其他细胞表面蛋白。在这项研究中，我们研究了SARS-COV的共受体的性质。通过利用可溶性SARS-COV尖峰蛋白和一种化学交联试剂，我们在Spike-ACE2复合物中鉴定了两个L型凝集素DC-SIGN和L-SIGN。在添加尖峰之前，ACE2与这些蛋白质形成复合物。尽管DC-SIGN和L-SIGN均未增加SARS-COV假病毒吸附到细胞表面，但这些凝集素促进了SARS-COV掺入细胞中。掺入需要DC-SIGN和L-SIGN的室内胞质区域，但不是ACE2的区域。这些结果表明，DC-SIGN和L-SIGN在SARS-COV感染中起作用的胞质内区域。

项目成果

Angiotensin II Type 1 Receptor Expression and Microvessel Density in Human Bladder Cancer

• DOI: 10.1016/j.urology.2010.11.002
• 发表时间: 2011-04-01
• 期刊: UROLOGY
• 影响因子: 2.1
• 作者: Shirotake, Suguru;Miyajima, Akira;Oya, Mototsugu
• 通讯作者: Oya, Mototsugu

C-terminal region of ErbB3 controls ErbB2/ErbB3 heterodimer signaling through ubiquitin-dependent receptor degradation

ErbB3 的 C 端区域通过泛素依赖性受体降解控制 ErbB2/ErbB3 异二聚体信号传导

• 发表时间: 2010
• 作者: 須賀淳子;菅村和夫;田中伸幸
• 通讯作者: 田中伸幸

HCV感染における小胞輸送タンパクHrsの役割

囊泡转运蛋白Hrs在HCV感染中的作用

• 发表时间: 2010
• 作者: 玉井恵一;田中伸幸;上野義之;下瀬川徹;菅村和夫
• 通讯作者: 菅村和夫

Regulation of interleukin-21 receptor expression and its signal transduction by WSB-2

WSB-2对白细胞介素21受体表达及其信号转导的调控

• DOI: 10.1016/j.bbrc.2010.01.004
• 发表时间: 2010
• 期刊: Biochem. Biophys. Res. Commun
• 作者: Nara H;Onoda T;Rahman M;Araki A;Juliana FM;Tanaka N and Asao H
• 通讯作者: Tanaka N and Asao H

Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting

• DOI: 10.1074/jbc.m110.191924
• 发表时间: 2011-04-29
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Amano, Yuji;Yamashita, Yuki;Takeshita, Toshikazu
• 通讯作者: Takeshita, Toshikazu