Analysis of genetic abnormality related to die invasion of oral cancer and its clinical appliance

口腔鳞癌pRb2/p130的免疫组化检查显示，pRb2/p130在许多分化良好的肿瘤和临床低恶性未发现转移灶的病例中均呈阳性染色。pRb2/p130蛋白的表达可能是口腔鳞状细胞癌患者预后的一个良好指标。口腔鳞状细胞癌的细胞粘附相关因子E-cadherin、α-catenin、βcatenin、上皮生长因子受体（Epithelial Growth Factor Receptor， BGFR）的免疫组化检查发现，这些因子的表达与局部转移的存在相关，这意味着这些因子在许多转移灶的病例中表达降低。增殖相关因子p53蛋白的表达与局部转移灶的存在无相关性，β-catenin在外显子3的tte区域未发现突变。在口腔癌细胞系中发现了p16INK4A、DAPK、DOC、MINT1、MINT2、MINT27和MINT31等基因的异常甲基化，在人舌癌组织中发现了p14ARF、p15INK4B、p16INK4A、DAPK、DOC、MINT1、MINT2、MINT27和MINT31等基因的异常甲基化。p14ARF的异常甲基化在许多舌癌中被发现。构建了rgd -纤维修饰的腺病毒载体，并通过该载体提高了体内外基因转导效率。在正常组织中，使用该载体不能识别基因转导。

Immunohistochemical examination of the cases with oral squamous cell carcinoma with pRb2/p130 showed that positive staining for pRb2/p130 was observed in many of the cases with well differentiated tumor and clinically low malignant cases in which a metastatic lesion was not found. Expresdion of pRb2/p130 protein may be a good prognostic indicator ui patients with oral squamous cell carcinoma.Imuunohistochemical examination of the cases with oral squamous cell carcinoma with E-cadherin, α-catenin, βcatenin, and Epithelial Growth Factor Receptor (BGFR), which are cell-adhesion related factors, revealed that die expressions of these factors were found to be correlated with die existence of the regional metastasis, meaning that these expressions were reduced in many of the cases with the metastatic lesion. Expression of p53 protein, which is proliferation-related factor, was not correlated with the existence of the regional metastatic lesion Mutation of β-catenin was not found in tte area of exon-3.In established cell lines derived from oral cancer aberrant methylation of the genes such as p16INK4A, DAPK, DOC, MINT1, MINT2, MINT27, and MINT31 was found and in human tongue carcinoma tissues that such as p14ARF, p15INK4B, p16INK4A, DAPK, DOC, MINT1, MINT2, MINT27, and MINT31 was found. Aberrant mefttylation of p14ARF was found in many of wefl differentiated tongue carcinomas.We constructed RGD-fiber modified adenovirus vector and by this vector gene transduction efficiency was enhanced in vivo and in vitro. In normal tissues, gene transduction was not recognized in case of using this vector.