Studies for amelioration of adipocyte function to control obesity and diabetes using food factors.

在这项研究中，我们证明了花青素（cyanidin； Cy或cyanidin 3-glucoside； C3G）在分离的大鼠脂肪细胞中具有独特的药理功能。在不激活PPARγ的情况下，花青素处理大鼠脂肪细胞增强脂联素和瘦素分泌，上调脂肪细胞特异性基因表达。添加花青素的小鼠白色脂肪组织中脂联素的基因表达也上调。作为一种可能的机制，AMP活化的蛋白激酶活化可能与这些变化有关，然而，施用花青素显著降低了AMP: ATP的比率。为了阐明其功能机制，我们进行了基因芯片分析。上调的基因包括脂质代谢和信号转导相关基因，基于基因表达谱，我们证实了C3G或Cy对脂肪细胞的激素敏感脂肪酶的上调和脂溶活性的增强，这些数据表明花青素具有独特的治疗优势，并且在预防肥胖和糖尿病方面也具有重要意义。

In this study, we demonstrated that anthocyanins (cyanidin ; Cy or cyanidin 3-glucoside ; C3G) have the potency of a unique pharmacological function in isolated rat adipocytes. Treated adipocytes with anthocyanins enhanced adiponectin and leptin secretion and up-regulated the adipocyte specific gene expression without activation of PPARγ in isolated rat adipocytes. The gene expression of adiponectin was also up-regulated in white adipose tissue in mice fed an anthocyanin supplemented diet. As one of the possible mechanisms, AMP-activated protein kinase activation would be associated with these changes, nevertheless, the AMP: ATP ratio was significantly decreased by administration of the anthocyanins. To clarify the mechanism of the function, GeneChip microarray analysis was carried out. The up-regulated genes include lipid metabolism and signal transduction-related genes Based on the gene expression profile, we demonstrated the up-regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy. These data suggest that anthocyanins have a potency of unique therapeutic advantage, and also have important implications for preventing obesity and diabetes.