Trigeminal nerve stimulation-induced c-Fos expression in trigeminal spinal nucleus

本研究的目的是探讨电刺激舌神经的单侧中央切断端是否产生三叉神经-副交感神经反射血管扩张两侧下唇和刺激三叉神经核复合体神经元在人工通气，颈迷走交感神经切除深度麻醉与乌拉坦大鼠。免疫组化检测c-Fos表达用于评估长时间舌神经刺激的影响。结果发现，以10 min间隔刺激一侧舌神经200 min，（i）刺激时引起下唇血流量持续增加，主要在同侧下唇;（ii）引起三叉神经脊核除口侧亚核外的所有亚核中c-Fos蛋白的表达，（iii）诱导同侧三叉神经极间亚核/尾侧亚核过渡带内c-Fos阳性神经元增多 ...更多信息 （4）与对照组比较，对侧三叉神经脊束核各亚核c-Fos表达均无统计学意义（P> 0.05）。这些结果表明，氨基甲酸乙酯可以是有用的调查的同时测量三叉神经副交感神经反射血管舒张和c-Fos的表达后，舌神经刺激。结合我们以往的研究结果，我们认为电刺激舌神经所产生的冲动接受三叉神经脊核内三叉神经极间亚核/尾侧亚核过渡带的投射后，会聚于同侧三叉神经脊核内的极间亚核/尾侧亚核过渡带和副交感神经血管扩张神经元、唾液核，通过耳神经节投射到下唇。少

The aim of the present study was to investigate whether electrical stimulation of the unilateral central cut ends of lingual nerve produces an trigeminal-parasympathetic reflex vasodilatation in both sides of the lower lips and stimulate neurons in the trigeminal nuclear complex in the artificially ventilated, cervically vagosympathectomized rats deeply anesthetized with urethane. Immunohistochemical detection of c-Fos expression was used to assess the impact of prolonged lingual nerve stimulation. It was found that the unilateral lingual nerve stimulation at 10 min intervals for 200 min (i)elicited consistent blood flow increases predominantly in the ipsilateral side of the lower lip when stimulated, (ii)evoked the more profound expression of c-Fos protein ipsilaterally in all subnuclei of trigeminal spinal nucleus except trigeminal subnucleus oralis, (iii)induced the greater number of c-Fos positive neurons in the ipsilateral trigeminal subnucleus interpolaris/caudalis transition zon … More e compared with the four other areas (trigeiminal subnucleus caudalis/upper cervical spinal cord transition zone, subnucleus caudalis, subnucleus interpolaris, subnucleus oralis) of trigeminal spinal nucleus, and (iv)did not induce a statistical significant increase in the number of c-Fos expression in all subnucleus of the trigeminal spinal nucleus of the contralateral side in comparison with the control rats. These results suggest that urethane can be useful for the investigation of the simultaneous measurements of the trigeminal-parasympathetic reflex vasodilatation and the c-Fos expression following the lingual nerve stimulation. The present studies together with our previous data, it is suggested that impulse elicited by electrical stimulation of the lingual nerve converge on the ipsilateral trigeminal subnucleus interpolaris/caudalis transition zone in the trigeminal spinal nucleus and the parasympathetic vasodilator neurons, salivatory nucleus, after receiving projection from the trigeminal subnucleus interpolaris/caudalis transition zone in the trigeminal spinal nucleus, project to the lower lip via the otic ganglion. Less