3D STED Super-resolution Confocal Microscope

我们为主申请人的研究实验室申请一种高分辨率显微镜，该显微镜可以可视化活细胞中的亚细胞结构，他最近接受了科隆大学的电话。拟议的仪器是新实验室的主要仪器，将专门用于研究。为了了解生命，研究细胞膜上的动态过程，如细胞内的扩散和分子相互作用是非常重要的。主要申请人是膜组织和动力学领域的国际领先专家，对他来说，在单分子水平上使用成像研究是必不可少的。该仪器将用于研究细胞死亡的调控，这对组织稳态、几种发育过程以及炎症性疾病和肿瘤形成具有关键意义。活细胞成像的新方法将在最近获得的欧洲研究委员会整合者资助和SFB1403和SFB1218的三个项目的背景下开发。建议的仪器将允许申请人在国际水平上继续成功的膜生物物理学研究。该仪器也将被CECAD研究中心肾实验室的成员定期使用，他们将特别使用该设备进行厚肾切片（150-500µm），以实现肾小球毛细血管的3D可视化。来自CECAD研究中心和科隆大学其他研究所的其他用户将在合作的基础上使用这一工具来解决其研究中的具体问题。

We apply for a high resolution microscope that allows the visualization of subcellular structures in living cells for the research laboratory of the main applicant, who recently accepted a call to the University of Cologne. The proposed instrumentation is the main instrument of the new laboratory and will be used exclusively for research. To understand life, it is very important to investigate dynamic processes in membranes like diffusion and molecular interactions directly in living cells. The main applicant is an international leading expert in the field of membrane organization and dynamics, for whom research using imaging at the single molecule level is essential. The instrument will be used to study the regulation of cell death, which is of critical relevance for tissue homeostasis, for several developmental processes as well as for inflammatory diseases and tumor formation. New methods for imaging in living cells will be developed in the context of a recently awarded Consolidator Grant of the European Research Council and three projects in SFB1403 and SFB1218. The proposed instrumentation will allow the applicant to continue successful research on membrane biophysics at the international level.The instrument will also be used on a regular basis by members of the Nephrolab in the CECAD Research Center who will use this device particularly with thick kidney sections (150-500 µm) to allow for 3D visualization of glomerular capillaries. Additional users from the CECAD Research Center and other Institutes of the University of Cologne will use this instrument on a collaborative basis to address specific questions in their research.