Super Resolution STED Microscopy at UNC

北卡罗来纳大学超分辨率 STED 显微镜

• 批准号: 10414283
• 负责人: Stephanie Gupton
• 金额: $ 60万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414283
• 关键词: 3-Dimensional; 5 year old; Area; Arts; Biology; Biomedical Research; Cardiac; Cell physiology; Cells; Cellular biology; Color; Communities; DNA; Developmental Biology; Disease; Electrons; Environment; Fluorescence; Funding; Image; Image Analysis; Institution; Laboratory Research; Light; Medical; Medical Research; Methods; Microscope; Microscopy; Neurobiology; North Carolina; Optics; Program Description; RNA; Research; Research Personnel; Research Project Grants; Resolution; Schools; Science; System; Techniques; United States National Institutes of Health; Universities; college; imaging facilities; instrument; instrumentation; live cell imaging; medical schools; optical imaging; protein transport; tau Proteins

PROGRAM DESCRIPTION This request is for funds to purchase a Leica Tau-Stimulated Emission Depletion (Tau-STED) microscope on the Stellaris 8 platform with 1 depletion line. This instrument will be housed within the Hooker Imaging Center (HIC) in the Department of Cell Biology and Physiology in the School of Medicine at the University of North Carolina at Chapel Hill. The HIC provides access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of North Carolina at Chapel Hill, including the School of Medicine, College of Arts and Sciences, and other professional schools on campus. Super-resolution microscopy is an essential advancement in optical microscopy techniques in biomedical research that has rapidly matured over the last 5 years. Currently on campus, there is a single microscope capable of true super resultion microscopy, to serve the over 350 biomedical research laboratories on campus, and this scope is more than five years old, and cannot perform super resolution in 3 dimensions, in level cell mode, or at distances > 2 µm from the coverslip. Recent dramatic improvements provided by the commercially available Leica Tau-STED microscopy bring higher resolution in all three dimensions, increased sensitivity, and increased suitability for live cell imaging by exploiting changes in fluorescence lifetime following stimulated emission depletion. Furthermore, the commercially available 775 depletion line allows facile and gentle gated two color super-resolution imaging, such that it can be easily deployed in a core environment such as the HIC and accessible to the variety of research projects and labs on campus. The need for this instrument is demonstrated throughout the application with several diverse NIH supported projects in fields including; Neurobiology, Cell Biology, Cardiac Biology Developmental Biology, cytoskeletal biology, basic and disease-oriented protein trafficking biology, RNA and DNA biology. The abilities provided by Tau-STED will transform our fundamental understanding of the biology of these many arenas. Since the HIC began serving the imaging community of UNC 14 years ago, it has become an integral part of the medical research community currently participates in research projects with more than 100 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The steering committee of the HIC, and the directors of non- overlapping imaging cores on campus together deem that acquisition of a Tau-STED system as an essential addition to the repertoire of instrumentation available to PHS funded users at the University, and that the HIC is the optimal core to house the scope for reasons described throughout the proposal.

项目描述 此请求旨在为购买 Leica Tau 受激发射损耗 (Tau-STED) 显微镜提供资金 具有 1 条耗尽线的 Stellaris 8 平台。该仪器将安装在胡克成像中心内 北方大学医学院细胞生物学和生理学系（HIC） 卡罗莱纳州在教堂山。 HIC 提供全方位的光和电子光学、图像分析和 北卡罗来纳大学教堂山分校所有研究小组的形态测量方法，包括 医学院、文理学院和其他专业学院均在校园内。超分辨率 显微镜是生物医学研究中光学显微镜技术的重要进步，它迅速 过去5年已经成熟。目前在校园里，有一台显微镜能够实现真正的超级结果 显微镜，为校园内 350 多个生物医学研究实验室提供服务，范围超过 五年前，无法在 3 维、水平单元模式或距离 > 2 µm 下执行超分辨率 从盖玻片上。市售 Leica Tau-STED 最近提供了显着的改进 显微镜在所有三个维度上都具有更高的分辨率、更高的灵敏度以及更高的活体适用性 通过利用受激发射损耗后荧光寿命的变化进行细胞成像。此外， 市售 775 耗尽线可轻松、温和地进行门控双色超分辨率成像， 这样它就可以很容易地部署在HIC等核心环境中，并可供各种应用程序访问 校园内的研究项目和实验室。整个应用程序都证明了对该仪器的需求 NIH 支持的多个不同领域的项目包括：神经生物学、细胞生物学、心脏生物学 发育生物学、细胞骨架生物学、基础和疾病导向的蛋白质运输生物学、RNA 和 DNA生物学。 Tau-STED 提供的能力将改变我们对生物学的基本理解 在这众多的竞技场中。自从 14 年前 HIC 开始为北卡罗来纳大学成像界提供服务以来，它已经成为 目前，医学研究界的一个重要组成部分参与了 100 多个研究项目 PHS 资助医疗领域内的团体，以及 PHS 支持的项目，包括其他领域的研究人员 部门和邻近机构。 HIC 指导委员会和非董事 校园内重叠的成像核心共同认为获取 Tau-STED 系统是必不可少的 除了大学 PHS 资助用户可用的仪器清单之外，HIC 还 出于提案中所述的原因，容纳范围的最佳核心。