Super Resolution STED Microscopy at UNC

北卡罗来纳大学超分辨率 STED 显微镜

• 批准号: 10414283
• 负责人: Stephanie Gupton
• 金额: $ 60万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414283
• 关键词: 3-Dimensional; 5 year old; Area; Arts; Biology; Biomedical Research; Cardiac; Cell physiology; Cells; Cellular biology; Color; Communities; DNA; Developmental Biology; Disease; Electrons; Environment; Fluorescence; Funding; Image; Image Analysis; Institution; Laboratory Research; Light; Medical; Medical Research; Methods; Microscope; Microscopy; Neurobiology; North Carolina; Optics; Program Description; RNA; Research; Research Personnel; Research Project Grants; Resolution; Schools; Science; System; Techniques; United States National Institutes of Health; Universities; college; imaging facilities; instrument; instrumentation; live cell imaging; medical schools; optical imaging; protein transport; tau Proteins

PROGRAM DESCRIPTION This request is for funds to purchase a Leica Tau-Stimulated Emission Depletion (Tau-STED) microscope on the Stellaris 8 platform with 1 depletion line. This instrument will be housed within the Hooker Imaging Center (HIC) in the Department of Cell Biology and Physiology in the School of Medicine at the University of North Carolina at Chapel Hill. The HIC provides access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of North Carolina at Chapel Hill, including the School of Medicine, College of Arts and Sciences, and other professional schools on campus. Super-resolution microscopy is an essential advancement in optical microscopy techniques in biomedical research that has rapidly matured over the last 5 years. Currently on campus, there is a single microscope capable of true super resultion microscopy, to serve the over 350 biomedical research laboratories on campus, and this scope is more than five years old, and cannot perform super resolution in 3 dimensions, in level cell mode, or at distances > 2 µm from the coverslip. Recent dramatic improvements provided by the commercially available Leica Tau-STED microscopy bring higher resolution in all three dimensions, increased sensitivity, and increased suitability for live cell imaging by exploiting changes in fluorescence lifetime following stimulated emission depletion. Furthermore, the commercially available 775 depletion line allows facile and gentle gated two color super-resolution imaging, such that it can be easily deployed in a core environment such as the HIC and accessible to the variety of research projects and labs on campus. The need for this instrument is demonstrated throughout the application with several diverse NIH supported projects in fields including; Neurobiology, Cell Biology, Cardiac Biology Developmental Biology, cytoskeletal biology, basic and disease-oriented protein trafficking biology, RNA and DNA biology. The abilities provided by Tau-STED will transform our fundamental understanding of the biology of these many arenas. Since the HIC began serving the imaging community of UNC 14 years ago, it has become an integral part of the medical research community currently participates in research projects with more than 100 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The steering committee of the HIC, and the directors of non- overlapping imaging cores on campus together deem that acquisition of a Tau-STED system as an essential addition to the repertoire of instrumentation available to PHS funded users at the University, and that the HIC is the optimal core to house the scope for reasons described throughout the proposal.

程序说明 此请求是要购买Leica Tau刺激的排放耗尽（Tau-sted）显微镜 具有1部部署线的Stellaris 8平台。该乐器将安置在妓女成像中心中 （HIC）在北大学医学院细胞生物学和生理学系 卡罗来纳州教堂山。 HIC可访问全范围的光和电子光学，图像分析和 北卡罗来纳大学教堂山分校的所有研究小组的形态学方法，包括 医学院，艺术与科学学院以及校园的其他专业学校。超分辨率 显微镜是生物医学研究中光学显微镜技术的重要进步，该技术已迅速 在过去的5年中成熟。目前在校园里，有一个能够真正超级结果的显微镜 显微镜，为校园的350多个生物医学研究实验室服务，此范围超过 五岁，不能在3维，在级别的单元模式下或距离> 2 µm的超级分辨率 从盖玻片上。最近可用的Leica Tau sted提供的巨大改进 显微镜在所有三个维度上带来更高的分辨率，提高灵敏度，并提高了实时的适用性 通过在刺激发射消耗后利用荧光寿命的变化来进行细胞成像。此外， 市售的775部署线允许柔和且柔和的门控两种颜色的超分辨率成像， 这样它可以轻松地部署在诸如HIC之类的核心环境中，并且可以访问多种多样 校园的研究项目和实验室。在整个应用程序中都表明了该工具的需求 在包括：神经生物学，细胞生物学，心脏生物学 发育生物学，细胞骨架生物学，碱性和面向疾病的蛋白质运输生物学，RNA和RNA DNA生物学。 Tau STED提供的能力将改变我们对生物学的基本理解 在这些众多领域。自从HIC开始为14年前的UNC成像社区服务以来，它已成为 目前，医学研究界不可或缺的一部分参与了100多个的研究项目 PHS在医疗区域内以及PHS中资助的小组与其他调查人员在其他方面支持其他项目 部门和邻近机构。 HIC的指导委员会和非 - 在校园中重叠的成像核心在一起，认为将tau系统作为必不可少的 除了大学的PHS资助用户可用的仪器曲目外，HIC是 由于整个提案中描述的原因，最佳的核心是容纳范围的最佳核心。