Super Resolution STED Microscopy at UNC

节目描述 此申请是为了购买Leica Tau-刺激发射损耗（Tau-STED）显微镜的资金， Stellaris 8平台，带1条耗尽线。该仪器将放置在Hooker成像中心内 (HIC)在北方大学医学院细胞生物学和生理学系， 查佩尔山的卡罗莱纳。HIC提供全方位的光和电子光学，图像分析， 在查佩尔山的北卡罗来纳州大学的所有研究小组，包括 医学院、文理学院和其他专业学校在校园内。超分辨率 显微镜是生物医学研究中光学显微镜技术的重要进步， 在过去的五年里成熟了。目前在校园里，有一个单一的显微镜能够真正的超级结果， 显微镜，为校园内350多个生物医学研究实验室提供服务，这一范围超过 5年前，无法在3维、水平单元模式或距离> 2 µm处执行超分辨率 从盖玻片上。市售Leica Tau-STED提供的最新显著改进 显微镜在所有三个维度上都有更高的分辨率，增加了灵敏度，并增加了对生命的适应性。 通过利用受激发射耗尽后荧光寿命的变化进行细胞成像。此外，委员会认为， 市售的775耗尽线允许容易和温和的选通双色超分辨率成像， 这样，它可以很容易地部署在核心环境中，如HIC，并可供各种 研究项目和校园实验室。在整个应用程序中证明了对该仪器的需要 与几个不同的NIH支持的项目领域，包括：神经生物学，细胞生物学，心脏生物学 发育生物学，细胞骨架生物学，基础和疾病导向蛋白质运输生物学，RNA和 DNA生物学Tau-STED提供的能力将改变我们对生物学的基本理解 在这么多的舞台上。自14年前HIC开始服务于美国的成像社区以来，它已成为 医学研究界的一个组成部分，目前参与了100多个研究项目， 公共卫生服务局资助的医疗领域内的团体，以及公共卫生服务局支持的项目与调查人员在其他领域， 部门和邻近机构。生境联盟指导委员会和非生境联盟理事会理事， 校园内重叠的成像核心共同认为， 除了仪器的剧目可供PHS资助的用户在大学，和HIC是 最佳核心，以容纳整个建议书中所述的原因范围。

PROGRAM DESCRIPTION This request is for funds to purchase a Leica Tau-Stimulated Emission Depletion (Tau-STED) microscope on the Stellaris 8 platform with 1 depletion line. This instrument will be housed within the Hooker Imaging Center (HIC) in the Department of Cell Biology and Physiology in the School of Medicine at the University of North Carolina at Chapel Hill. The HIC provides access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of North Carolina at Chapel Hill, including the School of Medicine, College of Arts and Sciences, and other professional schools on campus. Super-resolution microscopy is an essential advancement in optical microscopy techniques in biomedical research that has rapidly matured over the last 5 years. Currently on campus, there is a single microscope capable of true super resultion microscopy, to serve the over 350 biomedical research laboratories on campus, and this scope is more than five years old, and cannot perform super resolution in 3 dimensions, in level cell mode, or at distances > 2 µm from the coverslip. Recent dramatic improvements provided by the commercially available Leica Tau-STED microscopy bring higher resolution in all three dimensions, increased sensitivity, and increased suitability for live cell imaging by exploiting changes in fluorescence lifetime following stimulated emission depletion. Furthermore, the commercially available 775 depletion line allows facile and gentle gated two color super-resolution imaging, such that it can be easily deployed in a core environment such as the HIC and accessible to the variety of research projects and labs on campus. The need for this instrument is demonstrated throughout the application with several diverse NIH supported projects in fields including; Neurobiology, Cell Biology, Cardiac Biology Developmental Biology, cytoskeletal biology, basic and disease-oriented protein trafficking biology, RNA and DNA biology. The abilities provided by Tau-STED will transform our fundamental understanding of the biology of these many arenas. Since the HIC began serving the imaging community of UNC 14 years ago, it has become an integral part of the medical research community currently participates in research projects with more than 100 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The steering committee of the HIC, and the directors of non- overlapping imaging cores on campus together deem that acquisition of a Tau-STED system as an essential addition to the repertoire of instrumentation available to PHS funded users at the University, and that the HIC is the optimal core to house the scope for reasons described throughout the proposal.