The roles for biliary epithelial cells as antigen presenting cells or target cells in primary biliary cirrhosis

胆汁上皮细胞(BEC)是在肝移植过程中由病肝分化而来的细胞，表达CD40和Toll样受体(TLR)2、3和4。将自身抗原丙酮酸脱氢酶复合体E_2组分(PDC-E_2)163-176肽冲击BEC，并将PDC-E_2 163-176反应性T细胞克隆(TCC)与PDC-E_2反应性T细胞克隆(TCC)共染色，进行TCC扩增试验。在某些情况下，BEC预先与干扰素-γ、CD40L、TLR2、3或4L共同孵育。在所有病例中，BEC均不能增殖TCC。BEC经干扰素-γ、CD40L、TLR2、3、4配体刺激后仍不表达共刺激分子CD80或CD86。体外培养的BEC在干扰素-γ、TLR2、3、4配体的刺激下，产生趋化因子CXCL8、CCL2、CXCL9、CXCL10、CX3CL1和CXCL16。BEC自发产生CXCL8和CCL2。TLR3配体刺激后产生CXCL10、CX3CL1和CXCL1G。结论：BEC不具有抗原提呈细胞的作用，干扰素-γ刺激使BEC成为靶细胞，而其他刺激不影响BEC作为靶细胞。细胞，BEC自发地或与TLR3配体唯一地产生趋化因子。

Biliary epithelial cells-(BEC) which established from disease liver in liver transplantation from.Primary biliary cirrhosis expressed CD40 and Toll-like receptor (TLR)2,3 and 4. BEC were examined whether they have roles as antigen presenting cells. Auto antigen pyruvate dehydrogenase complex E2 component (PDC-E2) 163-176 peptide was pulsed to BEC, and PDC-E2 163-176 reactive T cell clones (TCC) were co-coltured with the peptide profsed BEC, an TCC profiferation assay were performed. In some cases, BEC were pre-incubated with IFN-γ, CD40 ligand, TLR2,3,or 4 ligand. In all cases BEC could not proliferate TCC. BEC could not express co-stimulation molecules, CD80 or CD86 even after stimulated with IFN-γ, CD40 ligand, TLR2,3,or 4 ligand.Next BEC were examined whether sensitivity as target cells are increased after stimulated with IFN-y, CD40 ligand, TLR2,3,or 4 ligand. BEC have roles as target cells after stimulated with IFN-y, on the other hand BEC did not have roles as target cells with other stimulation.Finally chemokine CXCL8,CCL2,CXCL9,CXCL10,CX3CL1 and CXCL16 production from BEC were examined with or without the stimulation of IFN-γ, TLR2,3,or 4 ligand. BEC produced CXCL8 and CCL2 spontaneously. CXCL10,CX3CL1 and CXCL1G were produced after the stimulation with TLR3 ligand. IFN-γ increased the production of such chemokines.In conclusion, BEC were clarified that they do not have roles as antigen presenting cells, that IFN-g stimulation turn BEC as target cells but other stimulation did not affect BEC as target. cells, and that BEC produce chemokines spontaneously or uniquely with TLR3 ligand.