Toxification of puffer and tetrodotoxin-associated proteins in liver

河豚的肝组织切片在体外积聚河豚毒素(TTX)，当与含有河豚毒素的最低基本培养基孵育时。在浓度为25μg Ttt/m l的河豚肝切片中，TTx在2 h时首次检测到3.9μg/g，48h时增加到15.0μg/g。河豚毒素在培养2小时后开始积累，48h时增加到15.0μg/g。即使在没有TTX的情况下进一步孵育48h，累积的TTX含量也没有减少。反之，在浓度为25μg TTx/ml的情况下，用25μg TTx/m l孵育的石斑鱼、六角鱼和小沙鱼的肝脏切片，即使在0.5h也能检测到3-4 Fg g/g的TTx。孵育48h后，TTX含量无明显变化。在不加TTX的情况下，进一步孵育48h不会降低TTX的含量。本文研究了转运系统抑制剂对赤潮鱼肝组织切片中TTX体外蓄积的影响。在河豚肝组织切片中添加对氨基马尿酸和肉碱可显著降低河豚肝组织切片中TTX的积累量，而添加四乙基铵则不显著，提示有机离子转运体参与了河豚肝组织中TTX的积聚。

The liver tissue slices of pufferfish accumulate in vitro tetrodotoxin (TTX), when incubated with minimum essential medium containing TTX. In the case of Takifugu rubripes liver slices incubated at a concentration of 25μg TTT/ml, TTX of 3.9μg/g was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was not decreased, even when the slices were further incubated without TTX for additional 48h. On the contrary, in the cases of the liver slices from parrot-bass Oplegnathus fasciatus, green ling Hexagrammos otakii and filefish Thamnaconus modestus incubated at a concentration of 25μg TTX/ml, TTX of 3-4μg/g was detected even at 0.5h. However, no significant change in TTX contents was recognized during the incubation for 48h. Further incubation of the filefish liver slices without TTX for additional 48h did not decrease the TTX content. It is unlikely that the liver slices of slices of filefish as well as pufferfish rapidly excrete TTX.Effect of inhibitors of transport system on in vitro accumulation of TTX in T.rubripes liver tissue slices was examined. Addition of p-aminohippuric acid and carnitine to the culture media significantly decreased in TTX content accumulated in the puffer liver tissue slices while that of tetraethylammonium did not, suggesting the involvement of an organic ion transporter in TTX accumulation in puffer liver.