Search for genes associated with genome copy number imbalance in human cancer

许多人类癌症表现出DNA非整倍体，DNA指数为1.3或更高。我们认为这是人类肿瘤的基本特征之一，但对DNA异倍体获得的机制却知之甚少。为了详细检测总基因组拷贝数的异常，并探讨与DNA非整倍体获得机制相关的基因，我们用比较基因组杂交的方法对一些肿瘤进行了分析。首席调查员和其他研究人员(WE)发现并报告了在DNA非整倍体的粘液型结直肠癌中，18P处反复发生的DNA拷贝数异常(获得)。此外，我们还报道了Aurora-A基因所在的DNA倍体20q13与中心体高扩增、拷贝数增加或肿瘤复发有关。除了他们之外，首席调查员还利用机会获得了有关实体肿瘤DNA非整倍体的出版物和日语的细胞仪DNA测量技术笔记。我们还尝试了其他实验，例如，使用培养细胞诱导DNA倍体异常或检测癌前病变中的基因组复制异常的实验。我们正在准备根据这些实验的结果发表论文。

Many of human carcinomas show DNA aneuploidy with DNA index 1.3 or more. We think that this is one of basic feature of human carcinoma, however, little is known about the mechanism of acquisition of DNA aneuploidy. For detecting overall genome copy number aberration in detail and approaching to genes associated with the mechanism of acquisition of DNA aneuploidy, some carcinomas have been analyzed by comparative genomic hybridizations. The head investigator and other researchers (we) found and reported that a recurrent DNA copy number aberration (gain) at 18p in mucinous type colorectal carcinoma with DNA aneuploidy. Furthermore, we have reported that centrosome hyperamplification associated with DNA ploidy, 20q13, on which the Aurora-A gene is located, copy number increasing or tumor recurrence in bladder cancer. In addition to them, the head investigator took chances for publications about DNA aneuploidy in solid tumors and technical notes for DNA measurement with cytometer in Japanese. We have tried other experiments, for example, an experiment to induce DNA ploidy abnormality using cultured cell or to detect genome copy aberrations in precancerous lesions. We are preparing for publishing based on results from these experiments.