Development of prediction method of industrial respiratory allergens and analysis of underlying mechanism in respiratory allergy

我们推进了工业场所低分子化学品呼吸敏感性预测试验的开发，以及哮喘动物模型的制作，用于哮喘病情的分析。因此，取得了以下结果。将细胞因子的产生模式作为预测皮肤致敏小鼠呼吸敏感性的指标是有益的。此外，它被证明能够使指数区分呼吸和皮肤敏感性。也就是说，通过评估IL-4或IFN-γ是否具有Th1和Th2型细胞因子的优势，可以区分呼吸和皮肤敏感性。提示血清总IgE抗体可作为小鼠气管内给药时的呼吸敏感性指标。我们开发了一种简单的气管内给药设备，使其能够高度定量给药。利用该装置，我们还成功地刺激了低分子化学物质（TDI和TMA）引起的小鼠呼吸道过敏。此外，还可以定量地从蛋白抗原到低分子化学物质，并比较在相同条件下的敏感性强度。

We have advanced the development of prediction test for the respiratory sensitivity of low molecular chemicals used in industrial places, and the producing of asthma model in animals for the analysis of asthma condition. Consequently, the following results were achieved.It was beneficial to use the production pattern of cytokines as a indicator for the prediction of the respiratory sensitivity in mice which were exposed cutaneously to sensitizations. Moreover, it was shown to enable the index to distinguish respiratory and skin sensitivity. That is, the respiratory and skin sensitivity were able to be distinguished by the evaluation whether IL-4 or IFN-γ, which are Th1 and the Th2 type cytokines, have an advantage.It was suggested that serum total IgE antibody could be used the index of respiratory sensitivity when test substances were administrated intratracheally in mice.We developed a simple equipment for the intratracheal administration, which enabled highly quantitative administering. Using the equipment, we also succeeded in the provocation of the respiratory allergy induced by low molecular chemicals (TDI and TMA)in the mouse. Further, it was possible to administer quantitatively from protein antigens to low molecular chemicals, and to compare the intense of sensitivity under the same condition.