The analysis of thymidine phospholyrase and mucin expression related to invasion and metastasis in pancreas cancer

我们发现胸苷磷酸化酶（TP）或膜结合型粘蛋白（MUC1）的过表达与各种类型肿瘤的血相转移或预后不良密切相关。胰腺癌中TP过表达的发生率较高。TP能促进细胞的运动和侵袭，抑制细胞凋亡。TP抑制剂抑制TP的表达，与癌细胞的侵袭转移密切相关。此外，我们发现TP与HIF-1途径有关，可避免细胞凋亡。MUC1的表达与胰腺癌的恶性生物学行为密切相关。我们通过肠型分泌黏液蛋白（MUC2）的表达报道了胰腺导管内乳头状-黏液性肿瘤的新分类。胰腺癌是实体瘤中最具侵袭性的癌症之一。根治性切除后复发率高。我们研究了胰腺癌中淋巴结微转移的存在。淋巴结微转移在pN0型胰腺癌中也很常见。临床意义应在大量患者中明确，特别是远处淋巴结微转移。免疫组化法检测胰腺癌组织中VEGF-C和-D的表达。VEGF-C和vegf -D过表达在肿瘤边缘部位的发生率明显高于肿瘤中心部位。边缘部分VEGF-C和-D均高表达的患者预后明显差于VEGF-C和-D均低表达的患者。通过活检标本检测VEGF-C和vegf -D的表达将有助于预测胰腺癌的恶性性质。

We found the close relationship between overexpression of thymidine phosphorylase(TP) or membrane-bound type mucin(MUC1) and hematogeneous metastasis or poor prognosis in various type of cancer. The incidence of TP overexpression was high in pancreatic cancer. Cell movement and invasion was promoted by TP, but apoptosis was suppressed by TP. TP was closely related to invasion and metastasis in cancer cell because TP inhibitor suppressed the expression of TP. Furthermore, we found that TP was related to the HIF-1 pathway in avoidance of apoptosis. MUC1 expression correlated well with the malignant biological behavior in pancreatic cancer. We reported the new classification of intraductal papillary-mucinous tumor in the pancreas by intestinal type secretory mucin(MUC2) expression.Pancreatic cancer is one of the most aggressive cancers in solid tumor. The high incidence of recurrent disease was found after curative resection. We examined the presence of lymph node micrometastasis in pancreatic cancer. Lymph node micrometastasis was highly found even in pN0 pancreatic cancer. The clinical significance should be clarified in the large number of patients, especially in the distant lymph node micrometastasis.The expression of VEGF-C and -D was immunohistochemically examined in pancreatic cancer. The incidence of VEGF-C and -D overexpression was significantly higher in the marginal portion than in the central portion of the tumor. Prognosis of patients with high expression of both VEGF-C and -D in the marginal portion was significantly poorer than that of patients with loe expression of both VEGF-C and -D. The examination of VEGF-C and -D expression by biopsy specimens will be useful to predict the malignant property in pancreatic cancer.