Study on the molecular mechanism of the secretion of pituitary hormofie

我们已经阐明了rab3B在生长激素（Gil）分泌中的作用，以及rab3B与可溶性n -乙基酰亚胺敏感因子附着蛋白受体（SNARE）蛋白之间的相互关系。共聚焦激光扫描显微镜观察rab3B和GH双免疫组化染色结果显示，ghrh处理大鼠rab3B免疫反应性升高，srlf处理大鼠rab3B免疫反应性降低。此外，共聚焦激光扫描显微镜观察SNAP-25、syntaxin和rab3B的双免疫组化染色显示，gi - irh处理大鼠中rab3B和SNARE蛋白共定位，srif处理大鼠中rab3B蛋白解离。这些结果表明rab3B在。垂体前叶细胞分泌GH， SNAP-25和syntaxin与rab3B一起作用于GH分泌的功能调控。另一项利用绿色荧光蛋白（GFP）的实验揭示了rab3B在生长激素分泌早期的作用以及SNARE蛋白在生长激素分泌中的作用。抑制rab3B可抑制GH的分泌。

We have elucidated the role of rab3B in growth hormone (Gil) secretion and the mutual relationship between rab3B and soluble N-ethylrnaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Confocal laser scanning microscopic observation of double immunohistochemical stainings for rab3B and GH revealed an increase of imrnunoreactivity for rab3B in GHRH-treated rats and a decrease of immunoreactivity for rab3B in SRLF-treated rats. Moreover, confocal laser scanning microscopic observation of double immunohistochemical stainings of SNAP-25, syntaxin and rab3B revealed co-localization of rab3B and the SNARE proteins in GI-IRH-treated rats, and dissociation in SRIF-treated rats. These results suggest that rab3B plays an important role in. Gil secretion from the anterior pituitary cells, and that SNAP-25 and syntaxin act alongside rab3B in the functional regulation of GH secretion. Another experiment using green fluorescent protein(GFP) has revealed the role of rab3B in the early stage of secretion of GH and the role of SNARE protein in the secretion of GH. The suppresion of rab3B is shown to inhibit the secretion of GH.