Direct administration therapy for irreversible peripheral vestibular disorder

我们评价了自由基清除剂依达拉奉对链霉素(SM)或AMPA1诱导的前庭功能障碍的影响。豚鼠右内耳采用30%丹参溶液渗透压泵给药24 h造成损伤。全身应用依达拉奉可改善损伤后3天和7天豚鼠的前庭-眼反射增益。局部应用依达拉奉可抑制豚鼠在病变后观察到的自发性眼球震颤的频率。用注射泵给豚鼠右内耳注入10 mM、50μL溶液5min，造成豚鼠右内耳病变。AMPA注射后可观察到自发的眼球震颤，同时注射AMPA受体拮抗剂CNQX可剂量依赖性地抑制眼球震颤的发生。局部应用依达拉奉也可抑制眼球震颤，早期(12h内)应用依达拉奉可改善损伤后1周的冷热刺激反应。结论：局部应用依达拉奉可能是治疗氨基糖苷类或谷氨酸兴奋性毒性所致前庭功能障碍的方法之一。

We evaluated the effects of edaravone, a free radical scavenger, on the vestibular disorder induced by streptomycin (SM) or AMPA1. Lesions were made in the guinea pig right inner ear by intracochlear administration of 30% SM for 24 h using osmotic pump. Systemic administration of edaravone improved vestibulo-ocular reflex gain in guinea pigs at 3 and 7 days after lesions were made. Local application of edaravone suppressed the frequency of spontaneous nystagmus observed in guinea pigs after lesions were made2. Lesions were made in the guinea pig right inner ear by infusion of AMPA (10 mM, 50 μl) for 5 min using syringe pump. After AMPA infusion, spontaneous nystagmus was observed, and simultaneous infusion of CNQX, a potent AMPA receptor antagonist, suppressed the frequency of nystagmus in a dose-dependent manner. Local application of edaravone also suppressed the nystagmus, furthermore, early (within 12 h) application of edaravone improved a response for caloric stimulation at 1 w after lesions were madeWe concluded that local application of edaravone may be one of the treatment for vestibular disorder caused by aminoglycoside or glutamate excitotoxicity