Trace element analysis, focused on contaminated elements, of oral mucosa of patents with oral lichen planus by PIXE method

1.本文采用粒子诱导x射线发射（PIXE）法对22例口腔扁平苔藓患者（olp组）和100例正常人（对照组）口腔黏膜微量元素含量进行了比较研究。两组口腔黏膜均检测到微量元素17种，污染元素11种。OLP组Se、Rb平均值明显低于对照组。对于30岁以上的女性，OLP组的Zn和Sr值明显高于对照组。其他必需元素和污染元素的平均值在各组之间没有显著差异，尽管在OLP组有更高的值的趋势。我们通过PIXE方法发现，被认为会引起过敏反应的d过渡元素Zn、Fe和Pd在OLP患者口腔黏膜中的值高于健康人。正常粘膜中的微量元素，超过800o的样品中检出Si、Cr、Mn、Fe、Ni、Cu、Zn和Se。在超痕量元素方面，90%以上的样品中检出了Br和Rb。正常口腔粘膜标本均检测到铝、铅。在Al的平均值中，39岁以下的女性高于40岁以上的女性。在铅的平均值中，40岁以上的男性高于40岁以上的女性。随着年龄的增长，Al的浓度有降低的趋势，而Pb的浓度有增加的趋势。金属义肢修复前后正常粘膜两亚组间Al、Pb浓度差异无统计学意义。

1.We made a Comparative study of the trace elements in oral mucosae of 22 patients with oral lichen planus (OLPgroup) and of 100 healthy persons (control group) by Particle Induced X-Ray Emission (PIXE) method.2.We detected 17 trace elements and 11 contaminated elements in the oral mucosae of both groups.3.The mean values of Se and Rb were significantly lower in the OLP group than in the control group. As for the females over 30 years old, the OLP group showed significantly higher values of Zn and Sr than the control group. The mean values of the other essential and contaminated elements did not differ significantly between the groups, although there was a trend toward higher values in the OLP group.4.We found by the PIXE method that Zn, Fe and Pd, which are d-transitional elements and considered to cause allergic reaction, showed higher values in the oral mucosae of the OLP patients than in those of the healthy persons.5. As to the trace elements in the normal mucosae, Si, Cr, Mn, Fe, Ni, Cu, Zn and Se were detected in greater than 800o of the samples. As to the ultratrace elements, Br and Rb were detected in greater than 90% of the samples.6.Al and Pb were detected in all the samples of normal oral mucosae. In the mean value of Al, the females under 39 years old showed a higher value than the females over 40 years old. In the mean value of Pb, the males over 40 years old showed a higher value than the females over 40 years old. The concentration of Al had a tendency to decrease with aging, while that of Pb had a tendency to increase. The concentration values of Al and Pb showed no significant differences between the two subgroups of normal mucosae with and without metallic prosthetic restorations.