Application of autotransfusion with -80℃ canine erythrocyte cryopreservation in veterinary medicine

在犬红细胞研究中，考察了-196℃和两步法（-196℃→80℃）冷冻保存的效果。将红细胞与含有羟乙基淀粉（HES）或甘油（GL）的冷冻保护液混合。将混合物装入氯乙烯制成的塑料袋中，在液氮中冷冻至-196℃。转移至-80℃深度冷冻室保存3个月。解冻后，红细胞不清洗，自行输血。1)犬红细胞在-196℃冷冻HES和GL后可自行输血，无副作用。2)将HES作为冷冻保护液，探索了两步法（-196℃→-80℃）大量冷冻保存犬红细胞的可能性。3)犬红细胞经-80℃HES低温保存3个月以上。在恢复率、渗透脆性试验、扫描电镜等指标上，各时期差异无统计学意义。因此，-80℃低温保存比液化保存能延长犬红细胞的保存时间。4)犬红细胞冷冻HES两步法可自行输注，无副作用。该方法可自行输血，在兽医学上具有实用价值。

In this canine erythrocytes study, the effect of -196℃ or two step method(-196℃→80℃) cryopreservation was examined. Erythrocytes were mixed with cryoprotective solution containing hydroxyethyl starch (HES) or glycerol (GL). This mixture was filled into plastic bag made of vinyl chloride and frozen to -196℃ in liquid nitrogen. It was transferred to -80℃ deepfreezer and was preserved for 3 month. After thawing, erythrocytes were not washed, and autotransfused.1)The canine erythrocytes frozen HES and GL at -196℃ could be autotransfused without side effects. The HES was considered to have a practical value rather than the GL.2)HES was used a cryoprotective solution, the possibility of the plentiful cryopreservation of canine erythrocytes at two step method (-196℃→-80℃) was found out.3)Canine erythrocytes were cryopreserved at -80℃ with HES for three months or more was found out. In recovery rate, osmotic fragility test and scanning electron microscopy, significant difference was not observed in each period. Therefore, -80℃ cryopreservation can preserve canine erythrocytes longer than liquefied preservation.4)The canine erythrocytes frozen HES at two step method could be autotransfused without side effects.This two step method that could be autotransfused, and was considered to have a practical value in veterinary medicine.