Search for new cell cycle inhibitors from marine organisms

海洋无脊椎动物已被证明是极其有效的化合物的丰富来源，这些化合物在抗肿瘤、抗炎、镇痛、免疫调节、过敏和抗病毒测试中显示出显著的活性。在本研究中，我们通过细胞毒性试验和流式细胞仪分析，从海洋生物中寻找新的细胞周期抑制物。泛素-蛋白酶体系统在多种细胞事件中发挥重要作用，包括细胞周期进程、转录调控和信号转导。然后，我们寻找泛素激活酶(E1)、泛素连接酶(E3)和蛋白酶体的抑制剂，发现了几种抑制这些酶的化合物。

The marine invertebrate has proven to be a rich source of extremely potent compounds that have demonstrated significant activities in antitumor, anti-inflammatory, analgesia, immunomodulation, allergy, and antiviral assays. In this study, we searched for new cell cycle inhibitors from marine organisms, by using assays of cytotoxicity and flow cytometric analysis. The ubiquitin-proteasome system plays important roles in various cellular events, including cell cycle progression, transcriptional regulation, and signal transduction. Then, we search for inhibitors of the ubiquitin-activating enzyme (E1), the ubiquitin ligase (E3), and the proteasome, and found several compounds which inhibit the enzymes.