Molecular biologic and histopathologic study on the expression status of Ca^<2+> binding protein genes in oral carcinoma

我们使用由2201个cDNA克隆组成的cDNA微阵列（AffysseGeneChip），包括48000个探针，检测了许多口腔癌、癌前病变和正常组织中Ca^2+结合蛋白基因的表达状态<TM>。真实的荧光定量PCR结果显示，与正常组织相比，27个基因在口腔癌组织中呈差异性过表达，83个基因呈差异性下调表达。内质网Ca^2+泵基因中ATP 2a家族基因表达被抑制。ATP 2b家族基因（作为细胞膜上的Ca^2+泵）和钙螯合蛋白-1基因（维持内质网中Ca^2+浓度）的表达也被检测到受到抑制。S100家族基因的表达也受到抑制，提示肿瘤的发生可能导致肿瘤细胞内多个系统的缓慢转换。

We examined many oral carcinoma, precancerous lesions, and normal tissues for the expression status of Ca^<2+> binding protein genes using our in-house cDNA microarray consisting 2201 cDNA clones Affymetrix GeneChip^<TM> consisting 48000 probes. The data of microarray analyses were confirmed by quantitative real time PCR.Twenty seven genes showed differential over-expression and 83 genes showed differential down expression in oral carcinoma, compared with those in normal tissue. Out of Ca^<2+> pump genes of endoplasmic reticulum ATP2a family genes were detected their suppressed expression. Expression of ATP2b family genes, which work as Ca^<2+> pump on the cell membrane, and calsequestrin-1 gene, which maintains the concentration of Ca^<2+> in the endplasmic reticulum, were also detected to be suppressed. The suppression of gene expression of S100 family genes, which play many roles, were also revealed.These results suggested that the carcinogenesis may resulting in slow switching of many systems in the carcinoma cells.