High accuracy function analysis of mitochondria with organelle adsorption method

目的：细胞器的功能虽然十分重要，但其功能测定方法的发展还不够。细胞器吸附法是近年来发展起来的一种测定细胞器活性的新方法。其特点是单个细胞器的测量和代谢产物从细胞器的快速分离。本研究用细胞器吸附法测定了线粒体的行为。结果与讨论：我们检测了解偶联蛋白2（UCP 2）、亲环素D（cyclophilin D）、神经节苷脂GD 3（ganglioside GD 3）、钙离子（Ca 2+）、呼吸底物对线粒体行为的影响，在国际期刊上发表了4篇原创论文，在国内期刊上发表了1篇综述。结果表明：1）UCP 2和亲环素D对氧化应激引起的线粒体损伤具有保护作用; 2）GD 3促进线粒体产生活性氧，并使线粒体逐渐去极化; 3）ADP、ATP和Mg ~（2+）稳定线粒体膜电位;这些结果为我们研究线粒体的行为提供了重要的信息。

Purpose:Developments of the method for measuring organelles' functions are not enough, although their functions are very important. Organelle adsorption method is a newly developed method which enables us to measure the activity of organelles adsorbed on the base. The characteristics are the measurements of individual organelles and the quick separation of the metabolites from the organelle. In this study, we measured mitochondrial behaviors with organelle adsorption method. Results and Discussions:We measured the effects of 1) the uncoupling protein 2 (UCP2), 2) cyclophilin D,3) ganglioside GD3, 4) Ca2+, 5) respiration substrates on mitochondrial behaviors, and published 4 original papers in international journal and one review in domestic journal. The results are 1) UCP2 and cyclophilin D protect mitochondria from oxidative stress-induced damage, 2) GD3 promotes the generation of reactive oxygen species from mitochondria and induces the gradual depolarization, 3) mitochondrial membrane potential are stabilized in the cell by the presence of ADP, ATP and Mg2+, 4) Ca2+ induced the swelling of depolarized mitochondria not but polarized mitochondria. These results provide us the important information on mitochondrial behaviors.