Diagnosis and monitoring of acute rejection after lung transplantation

肺移植术后急性期对急性排斥反应、再灌注损伤或肺部感染的准确诊断是困难的。我们探讨了流式细胞术检测外周血淋巴细胞表面抗原（CD3, CD4, CD8, CD25, CD69, CD45RA, CD45RO）的临床应用，以诊断移植后患者的状态。并讨论了抗cd25抗体的作用。我们发现病程稳定的患者CD4/CD8比值、CD25表达、CD69表达及CD45RA/RO比例无明显变化。CD28表达水平在移植后1个月内下降，与CD4亚群相比，这种趋势主要发生在CD8 T细胞中。间质性肺炎患者CD4 T细胞中CD25表达升高，CD8 T细胞中CD69表达升高。CD25抗体可抑制CD25的表达。

Accurate diagnosis of acute rejection, reperfusion injury, or pulmonary infection is difficult in the postoperative acute phase in the lung transplantation. We investigate the clinical utility of flow cytometry for surface antigen (CD3, CD4, CD8, CD25, CD69, CD45RA, CD45RO) on peripheral blood lymphocytes in order to diagnose the posttransplant status of patients. Also, the effect of anti-CD25 antibody administration was discussed. We found no evident change of CD4/CD8 ratio, CD25 expression, CD69 expression, and the proportion of CD45RA/RO in the patient with stable course. CD28 expression level decreased during the 1st month after transplant and this tendency was predominantly found in CD8 T cells as compared to CD4 subset. In a patient suffering interstitial pneumonia, increase of CD25 expression in CD4 T cells and increase of CD69 expression in CD8 T cells were shown. CD25 expression might be suppressed by CD25 antibody administration.