Application of three-dimensional scaffolds for regenerative medicine with the use of molecular precursor

本研究研究了含碳酸盐磷灰石（CA）薄涂层对柱状钛纤维网（孔隙率85%，孔径200-300 μm，直径2.8mm x长度6 mm）种植体骨小梁响应的影响。通过所谓的分子前驱体法沉积了薄的CA涂层。通过调节Ca/P=1.67，在Ca- edta /胺乙醇溶液中加入二磷酸二丁铵，得到分子前体溶液。将烧结好的圆柱形钛纤维网浸入分子前驱体溶液中，在600℃下焙烧2小时。浸渍烧成过程重复三次。电子探针显微分析（EPMA）证实，在钛纤维网内部可以沉积一层粘附的CA薄膜。将涂有ca的钛纤维网浸入模拟体液后，磷灰石晶体在钛纤维网上沉淀。将未涂布和ca涂布的钛纤维网分别插入兔左右股骨髁的小梁骨。组织学和组织形态学评估显示，植入12周后，ca涂层钛纤维网多孔区域内的骨形成量明显增加。目前的研究结果表明，薄的ca涂层钛网具有更好的骨导电性，将用于三维支架。

In the present study, the influence of thin carbonate-containing apatite (CA) coating on trabecular bone response to cylindrical titanium fiber mesh (porosity of 85 %, pore size of 200-300 μm, 2.8mm diameter x 6 mm length) implants was investigated. Thin CA coatings were deposited by the so-called molecular precursor method. Molecular precursor solution was obtained by adding dibutylammonium diphosphate salt to Ca-EDTA/ amine ethanol solution by adjusting Ca/P=1.67. Sintered cylindrical titanium fiber mesh was immersed into molecular precursor solution and then fired at 600 ℃ for 2 hr. The immersion and firing process was repeated three times. Electron probe microanalysis (EPMA) confirmed that an adherent thin CA film could be deposited on the inside of titanium fiber mesh. After the immersion of a CA-coated titanium fiber mesh in simulated body fluid, apatite crystals precipitated on the titanium fiber mesh. Uncoated and CA-coated titanium fiber mesh was inserted into the trabecular bone of the left and right femoral condyles of rabbits. Histological and histomorphometrical evaluation revealed a significantly greater amount of bone formation inside the porous area of the CA-coated titanium fiber mesh after 12 weeks of implantation. The present results suggested that a thin CA-coated titanium mesh has better osteoconductivity and will be useful for a three-dimensional scaffold.