High-resolution tandem mass spectrometer with liquid chromatography (UHPLC-QTOF-MS)

拟议的高分辨率混合质谱仪取代了现有的用于非靶向代谢物分析的UHPLC-QTOF-MS系统，即所谓的代谢物指纹图谱。该仪器由Göttingen分子生物科学中心(GZMB)植物生物化学部新陈代谢和脂体分析服务部操作。该服务股为GZMB、乔治·奥古斯特大学的各部门以及外部学术合作伙伴提供了分析小分子的综合基础设施，并提供有针对性和无针对性的方法。非定向代谢组分析提供了生物体在生物或非生物应激后或由于遗传或发育变化而产生的代谢状态的综合视图。在这种背景下，代谢物指纹图谱可以识别和表征代谢标志物或代谢途径，由于分析的深度很大，这些代谢标志物或途径在功能上与相应的影响因素有关。非定向代谢组分析的结果也被证明对产生可行的假说和开发新的科学问题非常有价值。建议的高分辨率混合质谱仪也将用于进一步发展我们的体外代谢组学方法，用于酶的功能注释。这种没有假设的方法结合了蛋白质生物化学和代谢物指纹图谱来确定未表征酶的底物-产物对，以描述它们的生理功能。此外，我们计划使用新的UHPLC-QTOF-MS系统，通过建立一个非靶向脂体分析平台来扩大我们在脂体分析方面的专业知识。这将补充现有的靶向分析方法，并能够识别以前未知的脂类标记物。UHPLC-QTOF-MS系统的更换将使我们能够进一步开发复杂的非靶向代谢组和脂体分析方法，并将其提供给科学界。

The proposed high-resolution hybrid mass spectrometer is a replacement for the existing UHPLC-QTOF-MS system for non-targeted metabolome analysis, so-called metabolite fingerprinting. The instrument is operated by the service unit Metabolome and Lipidome Analysis of the Department of Plant Biochemistry at the Göttingen Center for Molecular Biosciences (GZMB). The service unit provides a comprehensive infrastructure for the analysis of small molecules to the departments of the GZMB, the Georg-August-University, but also to external academic cooperation partners and offers targeted and non-targeted approaches. Undirected metabolome analysis provides a comprehensive view of the metabolic state of an organism after biotic or abiotic stress or as a consequence of genetic or developmental changes. In this context, metabolite fingerprinting allows the identification and characterization of metabolic markers or pathways that are functionally related to the corresponding influencing factors due to the large depth of analysis. The results of the undirected metabolome analysis have also proven to be very valuable for generating working hypotheses and developing new scientific questions. The proposed high-resolution hybrid mass spectrometer will also be used to further develop our ex vivo metabolomics approach for functional annotation of enzymes. This hypothesis-free approach combines protein biochemistry and metabolite fingerprinting to identify substrate-product pairs for uncharacterized enzymes to describe their physiological functions. In addition, we plan to use the new UHPLC-QTOF-MS system to expand our expertise in lipidome analysis by establishing a platform for non-targeted lipidome analysis. This will complement the existing method for targeted analysis and enable the identification of previously unknown lipid markers.The replacement of the UHPLC-QTOF-MS system will enable us to further develop sophisticated methods for non-targeted metabolome and lipidome analysis and make them available to the scientific community.