The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.

测定了牛细胞色素c氧化酶内在脂质的完整结构。His503将质子泵送过程与O_2还原过程耦合在一起。静止的氧化酶在O_2还原位点有过氧化物桥接。Fe_<a3>^<2+>处的O_2^-键被不连续的三个电子给能完全还原。h通道的水通道阻断了反向质子泄漏，提供了高效的能量耦合。获得了nadh -泛醌还原酶和f0f1atp酶的二维晶体。构建了适用于水溶液中任何蛋白质体系的高灵敏度红外仪器。

Complete structures of the intrinsic lipids in bovine cytochrome c oxidase were determined. His503 couples the proton-pumping process with O_2 reduction process. Resting oxidized enzyme has a peroxide bridged in the O_2 reduction site. The bound O_2^- at Fe_<a3>^<2+> is completely reduced by a non-consecutive three electron donation. The water-channel of the H-channel blocks the reverse proton leak to provide the highly effective energy coupling. Two dimensional crystals of NADH-ubiquinone reductase and F0F1ATPase were obtained. Highly sensitive infrared apparatus applicable for any protein system in aqueous solution was constructed.